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    Differential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains.

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    Authors
    Loessner, Holger
    Endmann, Anne
    Rohde, Manfred
    Curtiss, Roy
    Weiss, Siegfried
    Issue Date
    2006-12
    
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    Abstract
    Attenuated Salmonella enterica strains have been widely used as live carriers for vaccines and therapeutic molecules. Appropriate attenuation has been introduced into such bacteria for safety reasons and the improvement of strain properties. Here, we compared two strains that were rendered auxotroph for diaminopimelic acid or thymidine monophosphate precursors by deletion of the genes asd or thyA, respectively. Upon removal of the complementing compound from bacterial cultures, both strains quickly lose their property to form colonies. However, while the Deltaasd bacteria lysed almost immediately under such conditions, DeltathyA bacteria remained physically intact during the observation period. As a consequence, the Deltaasd bacteria released their intracellular content such as proteins or plasmids into the supernatant. In contrast, no intracellular component, either proteins or plasmids, could be recovered from the supernatants of DeltathyA bacteria upon depletion of thymidine. Thus, the release of macromolecules from the bacterial carrier occurs as a consequence of appropriate lethal attenuation. This might substitute for sophisticated secretion systems.
    Citation
    Differential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains. 2006, 265 (1):81-8 FEMS Microbiol. Lett.
    Affiliation
    Molecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. holger.loessner@helmholtz-hzi.de
    Journal
    FEMS microbiology letters
    URI
    http://hdl.handle.net/10033/15917
    DOI
    10.1111/j.1574-6968.2006.00470.x
    PubMed ID
    17034415
    Type
    Article
    Language
    en
    ISSN
    0378-1097
    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1574-6968.2006.00470.x
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