Show simple item record

dc.contributor.authorLoessner, Holger
dc.contributor.authorEndmann, Anne
dc.contributor.authorRohde, Manfred
dc.contributor.authorCurtiss, Roy
dc.contributor.authorWeiss, Siegfried
dc.date.accessioned2008-01-10T11:23:31Z
dc.date.available2008-01-10T11:23:31Z
dc.date.issued2006-12
dc.identifier.citationDifferential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains. 2006, 265 (1):81-8 FEMS Microbiol. Lett.en
dc.identifier.issn0378-1097
dc.identifier.pmid17034415
dc.identifier.doi10.1111/j.1574-6968.2006.00470.x
dc.identifier.urihttp://hdl.handle.net/10033/15917
dc.description.abstractAttenuated Salmonella enterica strains have been widely used as live carriers for vaccines and therapeutic molecules. Appropriate attenuation has been introduced into such bacteria for safety reasons and the improvement of strain properties. Here, we compared two strains that were rendered auxotroph for diaminopimelic acid or thymidine monophosphate precursors by deletion of the genes asd or thyA, respectively. Upon removal of the complementing compound from bacterial cultures, both strains quickly lose their property to form colonies. However, while the Deltaasd bacteria lysed almost immediately under such conditions, DeltathyA bacteria remained physically intact during the observation period. As a consequence, the Deltaasd bacteria released their intracellular content such as proteins or plasmids into the supernatant. In contrast, no intracellular component, either proteins or plasmids, could be recovered from the supernatants of DeltathyA bacteria upon depletion of thymidine. Thus, the release of macromolecules from the bacterial carrier occurs as a consequence of appropriate lethal attenuation. This might substitute for sophisticated secretion systems.
dc.language.isoenen
dc.subject.meshAmino Acid Oxidoreductasesen
dc.subject.meshDNA, Bacterialen
dc.subject.meshMicroscopy, Electronen
dc.subject.meshPlasmidsen
dc.subject.meshSalmonella Vaccinesen
dc.subject.meshSalmonella entericaen
dc.subject.meshThymidineen
dc.subject.meshThymidylate Synthaseen
dc.subject.meshVaccines, Attenuateden
dc.titleDifferential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains.en
dc.typeArticleen
dc.contributor.departmentMolecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. holger.loessner@helmholtz-hzi.deen
dc.identifier.journalFEMS microbiology lettersen
refterms.dateFOA2018-06-13T07:18:44Z
html.description.abstractAttenuated Salmonella enterica strains have been widely used as live carriers for vaccines and therapeutic molecules. Appropriate attenuation has been introduced into such bacteria for safety reasons and the improvement of strain properties. Here, we compared two strains that were rendered auxotroph for diaminopimelic acid or thymidine monophosphate precursors by deletion of the genes asd or thyA, respectively. Upon removal of the complementing compound from bacterial cultures, both strains quickly lose their property to form colonies. However, while the Deltaasd bacteria lysed almost immediately under such conditions, DeltathyA bacteria remained physically intact during the observation period. As a consequence, the Deltaasd bacteria released their intracellular content such as proteins or plasmids into the supernatant. In contrast, no intracellular component, either proteins or plasmids, could be recovered from the supernatants of DeltathyA bacteria upon depletion of thymidine. Thus, the release of macromolecules from the bacterial carrier occurs as a consequence of appropriate lethal attenuation. This might substitute for sophisticated secretion systems.


Files in this item

Thumbnail
Name:
Loessner et al_final.pdf
Size:
193.1Kb
Format:
PDF
Description:
original document
Thumbnail
Name:
figure-1 Loessner et al..ppt
Size:
124.5Kb
Format:
Microsoft PowerPoint
Description:
figure 1
Thumbnail
Name:
figure-2 Loessner et al..ppt
Size:
1.646Mb
Format:
Microsoft PowerPoint
Description:
figure 2
Thumbnail
Name:
figure-3 Loessner et al..ppt
Size:
1.655Mb
Format:
Microsoft PowerPoint
Description:
figure 3
Thumbnail
Name:
figure-4 Loessner et al..ppt
Size:
66.5Kb
Format:
Microsoft PowerPoint
Description:
figure 4
Thumbnail
Name:
figure-5 Loessner et al..ppt
Size:
33.5Kb
Format:
Microsoft PowerPoint
Description:
figure 5
Thumbnail
Name:
Supplementary Figure-1 Loessner ...
Size:
178.5Kb
Format:
Microsoft PowerPoint
Description:
complementary figure 1

This item appears in the following Collection(s)

Show simple item record