Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Weich, Herbert A
MetadataShow full item record
AbstractBACKGROUND: Lymphangiomas are neoplasias of childhood. Their etiology is unknown and a causal therapy does not exist. The recent discovery of highly specific markers for lymphatic endothelial cells (LECs) has permitted their isolation and characterization, but expression levels and stability of molecular markers on LECs from healthy and lymphangioma tissues have not been studied yet. We addressed this problem by profiling LECs from normal dermis and two children suffering from lymphangioma, and also compared them with blood endothelial cells (BECs) from umbilical vein, aorta and myometrial microvessels. METHODS: Lymphangioma tissue samples were obtained from two young patients suffering from lymphangioma in the axillary and upper arm region. Initially isolated with anti-CD31 (PECAM-1) antibodies, the cells were separated by FACS sorting and magnetic beads using anti-podoplanin and/or LYVE-1 antibodies. Characterization was performed by FACS analysis, immunofluorescence staining, ELISA and micro-array gene analysis. RESULTS: LECs from foreskin and lymphangioma had an almost identical pattern of lymphendothelial markers such as podoplanin, Prox1, reelin, cMaf and integrin-alpha1 and -alpha9. However, LYVE-1 was down-regulated and VEGFR-2 and R-3 were up-regulated in lymphangiomas. Prox1 was constantly expressed in LECs but not in any of the BECs. CONCLUSION: LECs from different sources express slightly variable molecular markers, but can always be distinguished from BECs by their Prox1 expression. High levels of VEGFR-3 and -2 seem to contribute to the etiology of lymphangiomas.
CitationElevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas. 2007, 7:105 BMC Cancer
AffiliationDepartment Gene Regulation and Differentiation, Helmholtz Centre for Infection Research, Braunschweig, Germany. firstname.lastname@example.org <email@example.com>
- The transcription factor Prox1 is a marker for lymphatic endothelial cells in normal and diseased human tissues.
- Authors: Wilting J, Papoutsi M, Christ B, Nicolaides KH, von Kaisenberg CS, Borges J, Stark GB, Alitalo K, Tomarev SI, Niemeyer C, Rössler J
- Issue date: 2002 Aug
- Isolation and characterization of lymphatic endothelial cells from human glossal lymphangioma.
- Authors: You L, Wu M, Chen Y, Xu G, Wei J, Li Q, Song A, Zhao L, Li S, Han Z, Zhou J, Lu Y, Wang S, Ma D, Meng L
- Issue date: 2010 Jan
- Similarities and differences of human and experimental mouse lymphangiomas.
- Authors: Kasten P, Schnöink G, Bergmann A, Papoutsi M, Buttler K, Rössler J, Weich HA, Wilting J
- Issue date: 2007 Oct
- Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.
- Authors: Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergün S
- Issue date: 2007 Dec 15
- Isolation and characterization of lymphatic microvascular endothelial cells from human tonsils.
- Authors: Garrafa E, Alessandri G, Benetti A, Turetta D, Corradi A, Cantoni AM, Cervi E, Bonardelli S, Parati E, Giulini SM, Ensoli B, Caruso A
- Issue date: 2006 Apr