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dc.contributor.authorNorgall, Susanne
dc.contributor.authorPapoutsi, Maria
dc.contributor.authorRössler, Jochen
dc.contributor.authorSchweigerer, Lothar
dc.contributor.authorWilting, Jörg
dc.contributor.authorWeich, Herbert A
dc.date.accessioned2008-01-17T13:50:03Z
dc.date.available2008-01-17T13:50:03Z
dc.date.issued2007
dc.identifier.citationElevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas. 2007, 7:105 BMC Canceren
dc.identifier.issn1471-2407
dc.identifier.pmid17584927
dc.identifier.doi10.1186/1471-2407-7-105
dc.identifier.urihttp://hdl.handle.net/10033/16274
dc.description.abstractBACKGROUND: Lymphangiomas are neoplasias of childhood. Their etiology is unknown and a causal therapy does not exist. The recent discovery of highly specific markers for lymphatic endothelial cells (LECs) has permitted their isolation and characterization, but expression levels and stability of molecular markers on LECs from healthy and lymphangioma tissues have not been studied yet. We addressed this problem by profiling LECs from normal dermis and two children suffering from lymphangioma, and also compared them with blood endothelial cells (BECs) from umbilical vein, aorta and myometrial microvessels. METHODS: Lymphangioma tissue samples were obtained from two young patients suffering from lymphangioma in the axillary and upper arm region. Initially isolated with anti-CD31 (PECAM-1) antibodies, the cells were separated by FACS sorting and magnetic beads using anti-podoplanin and/or LYVE-1 antibodies. Characterization was performed by FACS analysis, immunofluorescence staining, ELISA and micro-array gene analysis. RESULTS: LECs from foreskin and lymphangioma had an almost identical pattern of lymphendothelial markers such as podoplanin, Prox1, reelin, cMaf and integrin-alpha1 and -alpha9. However, LYVE-1 was down-regulated and VEGFR-2 and R-3 were up-regulated in lymphangiomas. Prox1 was constantly expressed in LECs but not in any of the BECs. CONCLUSION: LECs from different sources express slightly variable molecular markers, but can always be distinguished from BECs by their Prox1 expression. High levels of VEGFR-3 and -2 seem to contribute to the etiology of lymphangiomas.
dc.language.isoenen
dc.subject.meshAdolescenten
dc.subject.meshBiopsy, Needleen
dc.subject.meshCase-Control Studiesen
dc.subject.meshChilden
dc.subject.meshEndothelial Cellsen
dc.subject.meshEndothelium, Lymphaticen
dc.subject.meshEnzyme-Linked Immunosorbent Assayen
dc.subject.meshFemaleen
dc.subject.meshFluorescent Antibody Techniqueen
dc.subject.meshHumansen
dc.subject.meshLymphangiomaen
dc.subject.meshMaleen
dc.subject.meshPrognosisen
dc.subject.meshReference Valuesen
dc.subject.meshSampling Studiesen
dc.subject.meshSensitivity and Specificityen
dc.subject.meshSkin Neoplasmsen
dc.subject.meshTumor Cells, Cultureden
dc.subject.meshTumor Markers, Biologicalen
dc.subject.meshVascular Endothelial Growth Factor Receptor-2en
dc.subject.meshVascular Endothelial Growth Factor Receptor-3en
dc.titleElevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas.en
dc.typeArticleen
dc.contributor.departmentDepartment Gene Regulation and Differentiation, Helmholtz Centre for Infection Research, Braunschweig, Germany. susannenorgall@gmx.de <susannenorgall@gmx.de>en
dc.identifier.journalBMC canceren
refterms.dateFOA2018-06-12T17:58:24Z
html.description.abstractBACKGROUND: Lymphangiomas are neoplasias of childhood. Their etiology is unknown and a causal therapy does not exist. The recent discovery of highly specific markers for lymphatic endothelial cells (LECs) has permitted their isolation and characterization, but expression levels and stability of molecular markers on LECs from healthy and lymphangioma tissues have not been studied yet. We addressed this problem by profiling LECs from normal dermis and two children suffering from lymphangioma, and also compared them with blood endothelial cells (BECs) from umbilical vein, aorta and myometrial microvessels. METHODS: Lymphangioma tissue samples were obtained from two young patients suffering from lymphangioma in the axillary and upper arm region. Initially isolated with anti-CD31 (PECAM-1) antibodies, the cells were separated by FACS sorting and magnetic beads using anti-podoplanin and/or LYVE-1 antibodies. Characterization was performed by FACS analysis, immunofluorescence staining, ELISA and micro-array gene analysis. RESULTS: LECs from foreskin and lymphangioma had an almost identical pattern of lymphendothelial markers such as podoplanin, Prox1, reelin, cMaf and integrin-alpha1 and -alpha9. However, LYVE-1 was down-regulated and VEGFR-2 and R-3 were up-regulated in lymphangiomas. Prox1 was constantly expressed in LECs but not in any of the BECs. CONCLUSION: LECs from different sources express slightly variable molecular markers, but can always be distinguished from BECs by their Prox1 expression. High levels of VEGFR-3 and -2 seem to contribute to the etiology of lymphangiomas.


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