A German external quality survey of diagnostic microbiology of respiratory tract infections in patients with cystic fibrosis.
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
2008-01
Metadata
Show full item recordAbstract
BACKGROUND: The goal of this pilot study was to design an external quality assessment (EQA) scheme for German cystic fibrosis (CF) clinical microbiology laboratories. Therefore, a multicentre study of 18 German CF laboratories was performed to evaluate their proficiency in analyzing CF respiratory secretions. METHODS: Simulated clinical specimens containing a set of four frequent CF pathogens, namely two Pseudomonas aeruginosa strains differing in morphotype (mucoid versus non-mucoid) and resistotype, one Staphylococcus aureus strain and one Burkholderia multivorans strain, were distributed to each laboratory. Isolation, identification and antimicrobial susceptibility testing (AST) of any bacterial pathogen present and completion of a questionnaire about applied microbiological protocols were requested. RESULTS: Three of four strains were isolated and identified correctly by almost all laboratories. B. multivorans was once misidentified as Burkholderia cenocepacia. Fourteen laboratories failed to detect the second multidrug resistant P. aeruginosa isolate. AST errors occurred most often for P. aeruginosa 2 followed by B. multivorans, P. aeruginosa 1 and S. aureus. Evaluation of the questionnaires revealed major differences in cultivation and identification techniques applied by the participating laboratories. CONCLUSIONS: A periodical EQA programme for German CF laboratories and standardized microbiological procedures seem to be necessary to advance diagnostic microbiology employed on CF respiratory tract specimens and may help to improve anti-infective treatment and infection control practices for CF patients.Citation
A German external quality survey of diagnostic microbiology of respiratory tract infections in patients with cystic fibrosis. 2008, 7 (1):7-14notJ Cyst FibrosAffiliation
Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hanover, Germany.PubMed ID
17658302Type
ArticleISSN
1569-1993ae974a485f413a2113503eed53cd6c53
10.1016/j.jcf.2007.02.007
Scopus Count
Related articles
- EuroCareCF quality assessment of diagnostic microbiology of cystic fibrosis isolates.
- Authors: Hogardt M, Ulrich J, Riehn-Kopp H, Tümmler B
- Issue date: 2009 Nov
- Compliance of clinical microbiology laboratories in the United States with current recommendations for processing respiratory tract specimens from patients with cystic fibrosis.
- Authors: Zhou J, Garber E, Desai M, Saiman L
- Issue date: 2006 Apr
- Staphylococcus aureus and Pseudomonas aeruginosa Isolates from the Same Cystic Fibrosis Respiratory Sample Coexist in Coculture.
- Authors: Bernardy EE, Raghuram V, Goldberg JB
- Issue date: 2022 Aug 31
- Assessment of microbiological diagnostic procedures for respiratory specimens from cystic fibrosis patients in German laboratories by use of a questionnaire.
- Authors: Häfner L, Peters G, Kahl BC
- Issue date: 2014 Mar
- [Microbiological diagnosis of bronchopulmonary colonization-infection in cystic fibrosis].
- Authors: Oliver A, Alarcón T, Caballero E, Cantón R
- Issue date: 2009 Feb