Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Brakhage, Axel A
MetadataShow full item record
AbstractThe fungal pathogens Aspergillus fumigatus and Candida albicans are major health threats for immune-compromised patients. Normally, macrophages and neutrophil granulocytes phagocytose inhaled Aspergillus conidia in the two-dimensional (2-D) environment of the alveolar lumen or Candida growing in tissue microabscesses, which are composed of a three-dimensional (3-D) extracellular matrix. However, neither the cellular dynamics, the per-cell efficiency, the outcome of this interaction, nor the environmental impact on this process are known. Live imaging shows that the interaction of phagocytes with Aspergillus or Candida in 2-D liquid cultures or 3-D collagen environments is a dynamic process that includes phagocytosis, dragging, or the mere touching of fungal elements. Neutrophils and alveolar macrophages efficiently phagocytosed or dragged Aspergillus conidia in 2-D, while in 3-D their function was severely impaired. The reverse was found for phagocytosis of Candida. The phagocytosis rate was very low in 2-D, while in 3-D most neutrophils internalized multiple yeasts. In competitive assays, neutrophils primarily incorporated Aspergillus conidia in 2-D and Candida yeasts in 3-D despite frequent touching of the other pathogen. Thus, phagocytes show activity best in the environment where a pathogen is naturally encountered. This could explain why "delocalized" Aspergillus infections such as hematogeneous spread are almost uncontrollable diseases, even in immunocompetent individuals.
CitationEnvironmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans. 2007, 3 (2):e13 PLoS Pathog.
AffiliationDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany.
The following license files are associated with this item:
- Murine Intraepithelial Dendritic Cells Interact With Phagocytic Cells During Aspergillus fumigatus-Induced Inflammation.
- Authors: Bogorodskiy AO, Bolkhovitina EL, Gensch T, Troyanova NI, Mishin AV, Okhrimenko IS, Braun A, Spies E, Gordeliy VI, Sapozhnikov AM, Borshchevskiy VI, Shevchenko MA
- Issue date: 2020
- Phagocyte responses towards Aspergillus fumigatus.
- Authors: Hasenberg M, Behnsen J, Krappmann S, Brakhage A, Gunzer M
- Issue date: 2011 Jun
- Elimination of Aspergillus fumigatus conidia from the airways of mice with allergic airway inflammation.
- Authors: Shevchenko MA, Bolkhovitina EL, Servuli EA, Sapozhnikov AM
- Issue date: 2013 Jul 27
- Interaction of phagocytes with filamentous fungi.
- Authors: Brakhage AA, Bruns S, Thywissen A, Zipfel PF, Behnsen J
- Issue date: 2010 Aug
- Mechanisms of resistance of Aspergillus fumigatus Conidia to killing by neutrophils in vitro.
- Authors: Levitz SM, Diamond RD
- Issue date: 1985 Jul