Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans.
dc.contributor.author | Behnsen, Judith | |
dc.contributor.author | Narang, Priyanka | |
dc.contributor.author | Hasenberg, Mike | |
dc.contributor.author | Gunzer, Frank | |
dc.contributor.author | Bilitewski, Ursula | |
dc.contributor.author | Klippel, Nina | |
dc.contributor.author | Rohde, Manfred | |
dc.contributor.author | Brock, Matthias | |
dc.contributor.author | Brakhage, Axel A | |
dc.contributor.author | Gunzer, Matthias | |
dc.date.accessioned | 2008-02-26T14:15:58Z | |
dc.date.available | 2008-02-26T14:15:58Z | |
dc.date.issued | 2007-02 | |
dc.identifier.citation | Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans. 2007, 3 (2):e13 PLoS Pathog. | en |
dc.identifier.issn | 1553-7374 | |
dc.identifier.pmid | 17274685 | |
dc.identifier.doi | 10.1371/journal.ppat.0030013 | |
dc.identifier.uri | http://hdl.handle.net/10033/19196 | |
dc.description.abstract | The fungal pathogens Aspergillus fumigatus and Candida albicans are major health threats for immune-compromised patients. Normally, macrophages and neutrophil granulocytes phagocytose inhaled Aspergillus conidia in the two-dimensional (2-D) environment of the alveolar lumen or Candida growing in tissue microabscesses, which are composed of a three-dimensional (3-D) extracellular matrix. However, neither the cellular dynamics, the per-cell efficiency, the outcome of this interaction, nor the environmental impact on this process are known. Live imaging shows that the interaction of phagocytes with Aspergillus or Candida in 2-D liquid cultures or 3-D collagen environments is a dynamic process that includes phagocytosis, dragging, or the mere touching of fungal elements. Neutrophils and alveolar macrophages efficiently phagocytosed or dragged Aspergillus conidia in 2-D, while in 3-D their function was severely impaired. The reverse was found for phagocytosis of Candida. The phagocytosis rate was very low in 2-D, while in 3-D most neutrophils internalized multiple yeasts. In competitive assays, neutrophils primarily incorporated Aspergillus conidia in 2-D and Candida yeasts in 3-D despite frequent touching of the other pathogen. Thus, phagocytes show activity best in the environment where a pathogen is naturally encountered. This could explain why "delocalized" Aspergillus infections such as hematogeneous spread are almost uncontrollable diseases, even in immunocompetent individuals. | |
dc.language.iso | en | en |
dc.relation.url | http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17274685 | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Aspergillus fumigatus | en |
dc.subject.mesh | Candida albicans | en |
dc.subject.mesh | Cell Movement | en |
dc.subject.mesh | Cells, Cultured | en |
dc.subject.mesh | Dendritic Cells | en |
dc.subject.mesh | Environment | en |
dc.subject.mesh | Humans | en |
dc.subject.mesh | Luminescent Proteins | en |
dc.subject.mesh | Mice | en |
dc.subject.mesh | Mice, Inbred BALB C | en |
dc.subject.mesh | Neutrophils | en |
dc.subject.mesh | Phagocytes | en |
dc.subject.mesh | Phagocytosis | en |
dc.subject.mesh | Promoter Regions (Genetics) | en |
dc.title | Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans. | en |
dc.type | Article | en |
dc.contributor.department | Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany. | en |
dc.identifier.journal | PLoS pathogens | en |
refterms.dateFOA | 2018-06-13T19:47:49Z | |
html.description.abstract | The fungal pathogens Aspergillus fumigatus and Candida albicans are major health threats for immune-compromised patients. Normally, macrophages and neutrophil granulocytes phagocytose inhaled Aspergillus conidia in the two-dimensional (2-D) environment of the alveolar lumen or Candida growing in tissue microabscesses, which are composed of a three-dimensional (3-D) extracellular matrix. However, neither the cellular dynamics, the per-cell efficiency, the outcome of this interaction, nor the environmental impact on this process are known. Live imaging shows that the interaction of phagocytes with Aspergillus or Candida in 2-D liquid cultures or 3-D collagen environments is a dynamic process that includes phagocytosis, dragging, or the mere touching of fungal elements. Neutrophils and alveolar macrophages efficiently phagocytosed or dragged Aspergillus conidia in 2-D, while in 3-D their function was severely impaired. The reverse was found for phagocytosis of Candida. The phagocytosis rate was very low in 2-D, while in 3-D most neutrophils internalized multiple yeasts. In competitive assays, neutrophils primarily incorporated Aspergillus conidia in 2-D and Candida yeasts in 3-D despite frequent touching of the other pathogen. Thus, phagocytes show activity best in the environment where a pathogen is naturally encountered. This could explain why "delocalized" Aspergillus infections such as hematogeneous spread are almost uncontrollable diseases, even in immunocompetent individuals. |