High-resolution X-ray structure of the trimeric Scar/WAVE-complex precursor Brk1.
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AbstractThe Scar/WAVE-complex links upstream Rho-GTPase signaling to the activation of the conserved Arp2/3-complex. Scar/WAVE-induced and Arp2/3-complex-mediated actin nucleation is crucial for actin assembly in protruding lamellipodia to drive cell migration. The heteropentameric Scar/WAVE-complex is composed of Scar/WAVE, Abi, Nap, Pir and a small polypeptide Brk1/HSPC300, and recent work suggested that free Brk1 serves as a homooligomeric precursor in the assembly of this complex. Here we characterized the Brk1 trimer from Dictyostelium by analytical ultracentrifugation and gelfiltration. We show for the first time its dissociation at concentrations in the nanomolar range as well as an exchange of subunits within different DdBrk1 containing complexes. Moreover, we determined the three-dimensional structure of DdBrk1 at 1.5 Å resolution by X-ray crystallography. Three chains of DdBrk1 are associated with each other forming a parallel triple coiled-coil bundle. Notably, this structure is highly similar to the heterotrimeric α-helical bundle of HSPC300/WAVE1/Abi2 within the human Scar/WAVE-complex. This finding, together with the fact that Brk1 is collectively sandwiched by the remaining subunits and also constitutes the main subunit connecting the triple-coil domain of the HSPC300/WAVE1/Abi2/ heterotrimer to Sra1(Pir1), implies a critical function of this subunit in the assembly process of the entire Scar/WAVE-complex.
CitationHigh-resolution X-ray structure of the trimeric Scar/WAVE-complex precursor Brk1. 2011, 6 (6):e21327 PLoS ONE
AffiliationInstitute for Biophysical Chemistry, Hannover Medical School, Hannover, Germany.
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- Differential functions of WAVE regulatory complex subunits in the regulation of actin-driven processes.
- Authors: Litschko C, Linkner J, Brühmann S, Stradal TEB, Reinl T, Jänsch L, Rottner K, Faix J
- Issue date: 2017 Dec
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- Authors: Ibarra N, Pollitt A, Insall RH
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- The N-terminus of Dictyostelium Scar interacts with Abi and HSPC300 and is essential for proper regulation and function.
- Authors: Caracino D, Jones C, Compton M, Saxe CL 3rd
- Issue date: 2007 May
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- Authors: Blagg SL, Insall RH
- Issue date: 2004 Dec