Show simple item record

dc.contributor.authorKim, Hyun-Soo
dc.contributor.authorGolyshin, Peter N
dc.contributor.authorTimmis, Kenneth N
dc.date.accessioned2008-03-04T15:10:31Z
dc.date.available2008-03-04T15:10:31Z
dc.date.issued2007-11
dc.identifier.citationCharacterization and role of a metalloprotease induced by chitin in Serratia sp. KCK. 2007, 34 (11):715-21 J. Ind. Microbiol. Biotechnol.en
dc.identifier.issn1367-5435
dc.identifier.pmid17668255
dc.identifier.doi10.1007/s10295-007-0245-1
dc.identifier.urihttp://hdl.handle.net/10033/19693
dc.description.abstractA metalloprotease induced by chitin in a new chitinolytic bacterium Serratia sp. Strain KCK was purified and characterized. Compared with other Serratia enzymes, it exhibited a rather broad pH activity range (pH 5.0-8.0), and thermostability. The cognate ORF, mpr, was cloned and expressed. Its deduced amino acid sequence showed high similarity to those of bacterial zinc-binding metalloproteases and a well-conserved serralysin family motif. Pretreatment of chitin with the Mpr protein promoted chitin degradation by chitinase A, which suggests that Mpr participates in, and facilitates, chitin degradation by this microorganism.
dc.language.isoenen
dc.titleCharacterization and role of a metalloprotease induced by chitin in Serratia sp. KCK.en
dc.typeArticleen
dc.contributor.departmentDepartment of Environmental Microbiology, The Helmholtz Center for Infection Research, Braunschweig, Germany. hyun1006@korea.ac.kren
dc.identifier.journalJournal of industrial microbiology & biotechnologyen
refterms.dateFOA2018-06-12T21:30:19Z
html.description.abstractA metalloprotease induced by chitin in a new chitinolytic bacterium Serratia sp. Strain KCK was purified and characterized. Compared with other Serratia enzymes, it exhibited a rather broad pH activity range (pH 5.0-8.0), and thermostability. The cognate ORF, mpr, was cloned and expressed. Its deduced amino acid sequence showed high similarity to those of bacterial zinc-binding metalloproteases and a well-conserved serralysin family motif. Pretreatment of chitin with the Mpr protein promoted chitin degradation by chitinase A, which suggests that Mpr participates in, and facilitates, chitin degradation by this microorganism.


Files in this item

Thumbnail
Name:
Kim et al_final.pdf
Size:
216.9Kb
Format:
PDF
Description:
original manuscript
Thumbnail
Name:
Kim et al_Fig.ppt
Size:
102Kb
Format:
Microsoft PowerPoint
Description:
figures

This item appears in the following Collection(s)

Show simple item record