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dc.contributor.authorAmelung, Silva
dc.contributor.authorNerlich, Andreas
dc.contributor.authorRohde, Manfred
dc.contributor.authorSpellerberg, Barbara
dc.contributor.authorCole, Jason N
dc.contributor.authorNizet, Victor
dc.contributor.authorChhatwal, Gursharan S
dc.contributor.authorTalay, Susanne R
dc.date.accessioned2012-02-29T13:58:03Z
dc.date.available2012-02-29T13:58:03Z
dc.date.issued2011-08
dc.identifier.citationThe FbaB-type fibronectin-binding protein of Streptococcus pyogenes promotes specific invasion into endothelial cells. 2011, 13 (8):1200-11 Cell. Microbiol.en
dc.identifier.issn1462-5822
dc.identifier.pmid21615663
dc.identifier.doi10.1111/j.1462-5822.2011.01610.x
dc.identifier.urihttp://hdl.handle.net/10033/213571
dc.description.abstractInvasive serotype M3 Streptococcus pyogenes are among the most frequently isolated organisms from patients suffering from invasive streptococcal disease and have the potential to invade primary human endothelial cells (EC) via a rapid and efficient mechanism. FbaB protein, the fibronectin-binding protein expressed by M3 S. pyogenes, was herein identified as a potent invasin for EC. By combining heterologous gene expression with allelic replacement, we demonstrate that FbaB is essential and sufficient to trigger EC invasion via a Rac1-dependent phagocytosis-like uptake. FbaB-mediated uptake follows the classical endocytic pathway with lysosomal destination. FbaB is demonstrated to be a streptococcal invasin exhibiting EC tropism. FbaB thus initiates a process that may contribute to the deep tissue tropism and spread of invasive S. pyogenes isolates into the vascular EC lining.
dc.language.isoenen
dc.subject.meshAdhesins, Bacterialen
dc.subject.meshCarrier Proteinsen
dc.subject.meshCells, Cultureden
dc.subject.meshEndocytosisen
dc.subject.meshEndothelial Cellsen
dc.subject.meshFibronectinsen
dc.subject.meshHost-Pathogen Interactionsen
dc.subject.meshHumansen
dc.subject.meshStreptococcus pyogenesen
dc.subject.meshVirulence Factorsen
dc.subject.meshrac1 GTP-Binding Proteinen
dc.titleThe FbaB-type fibronectin-binding protein of Streptococcus pyogenes promotes specific invasion into endothelial cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medical Microbiology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.en
dc.identifier.journalCellular microbiologyen
refterms.dateFOA2012-08-15T00:00:00Z
html.description.abstractInvasive serotype M3 Streptococcus pyogenes are among the most frequently isolated organisms from patients suffering from invasive streptococcal disease and have the potential to invade primary human endothelial cells (EC) via a rapid and efficient mechanism. FbaB protein, the fibronectin-binding protein expressed by M3 S. pyogenes, was herein identified as a potent invasin for EC. By combining heterologous gene expression with allelic replacement, we demonstrate that FbaB is essential and sufficient to trigger EC invasion via a Rac1-dependent phagocytosis-like uptake. FbaB-mediated uptake follows the classical endocytic pathway with lysosomal destination. FbaB is demonstrated to be a streptococcal invasin exhibiting EC tropism. FbaB thus initiates a process that may contribute to the deep tissue tropism and spread of invasive S. pyogenes isolates into the vascular EC lining.


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