Identification of tumor-specific Salmonella Typhimurium promoters and their regulatory logic.
Leschner et al_final.pdf
Open Access publication
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Deyneko, Igor V
MetadataShow full item record
AbstractConventional cancer therapies are often limited in effectiveness and exhibit strong side effects. Therefore, alternative therapeutic strategies are demanded. The employment of tumor-colonizing bacteria that exert anticancer effects is such a novel approach that attracts increasing attention. For instance, Salmonella enterica serovar Typhimurium has been used in many animal tumor models as well as in first clinical studies. These bacteria exhibit inherent tumoricidal effects. In addition, they can be used to deliver therapeutic agents. However, bacterial expression has to be restricted to the tumor to prevent toxic substances from harming healthy tissue. Therefore, we screened an S. Typhimurium promoter-trap library to identify promoters that exclusively drive gene expression in the cancerous tissue. Twelve elements could be detected that show reporter gene expression in tumors but not in spleen and liver. In addition, a DNA motif was identified that appears to be necessary for tumor specificity. Now, such tumor-specific promoters can be used to safely express therapeutic proteins by tumor-colonizing S. Typhimurium directly in the neoplasia.
CitationIdentification of tumor-specific Salmonella Typhimurium promoters and their regulatory logic. 2012, 40 (7):2984-94 Nucleic Acids Res.
AffiliationMolecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124 Braunschweig, Germany. email@example.com
JournalNucleic acids research
The following license files are associated with this item:
- Remote control of tumour-targeted Salmonella enterica serovar Typhimurium by the use of L-arabinose as inducer of bacterial gene expression in vivo.
- Authors: Loessner H, Endmann A, Leschner S, Westphal K, Rohde M, Miloud T, Hämmerling G, Neuhaus K, Weiss S
- Issue date: 2007 Jun
- Salmonella enterica Serovar Typhimurium HtrA: regulation of expression and role of the chaperone and protease activities during infection.
- Authors: Lewis C, Skovierova H, Rowley G, Rezuchova B, Homerova D, Stevenson A, Spencer J, Farn J, Kormanec J, Roberts M
- Issue date: 2009 Mar
- Efficiency of conditionally attenuated Salmonella enterica serovar Typhimurium in bacterium-mediated tumor therapy.
- Authors: Frahm M, Felgner S, Kocijancic D, Rohde M, Hensel M, Curtiss R 3rd, Erhardt M, Weiss S
- Issue date: 2015 Apr 14
- Tumor-targeting amino acid auxotrophic Salmonella typhimurium.
- Authors: Hoffman RM
- Issue date: 2009 Sep
- Proteomic screening of anaerobically regulated promoters from Salmonella and its antitumor applications.
- Authors: Chen J, Wei D, Zhuang H, Qiao Y, Tang B, Zhang X, Wei J, Fang S, Chen G, Du P, Huang X, Jiang W, Hu Q, Hua ZC
- Issue date: 2011 Jun