Directing neuronal cell growth on implant material surfaces by microstructuring.
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Reich, UtaFadeeva, Elena
Warnecke, Athanasia
Paasche, Gerrit
Müller, Peter
Chichkov, Boris
Stöver, Timo
Lenarz, Thomas
Reuter, Günter
Issue Date
2012-05
Metadata
Show full item recordAbstract
For best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants.Citation
Directing neuronal cell growth on implant material surfaces by microstructuring. 2012, 100 (4):940-7 J. Biomed. Mater. Res. Part B Appl. Biomater.Affiliation
Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.PubMed ID
22287482Type
ArticleLanguage
enISSN
1552-4981ae974a485f413a2113503eed53cd6c53
10.1002/jbm.b.32656
Scopus Count
The following license files are associated with this item:
Related articles
- Differential fine-tuning of cochlear implant material-cell interactions by femtosecond laser microstructuring.
- Authors: Reich U, Mueller PP, Fadeeva E, Chichkov BN, Stoever T, Fabian T, Lenarz T, Reuter G
- Issue date: 2008 Oct
- Interaction of spiral ganglion neuron processes with alloplastic materials in vitro(1).
- Authors: Brors D, Aletsee C, Schwager K, Mlynski R, Hansen S, Schäfers M, Ryan AF, Dazert S
- Issue date: 2002 May
- [Growth behavior of spiral ganglion explants on cochlear implant electrodes and their materials].
- Authors: Hansen S, Mlynski R, Volkenstein S, Stark T, Schwaab M, Dazert S, Brors D
- Issue date: 2009 Apr
- Strategy towards independent electrical stimulation from cochlear implants: Guided auditory neuron growth on topographically modified nanocrystalline diamond.
- Authors: Cai Y, Edin F, Jin Z, Alexsson A, Gudjonsson O, Liu W, Rask-Andersen H, Karlsson M, Li H
- Issue date: 2016 Feb
- Scaling limitations of laser-fabricated nerve electrode arrays.
- Authors: Henle C, Schuettler M, Ordonez JS, Stieglitz T
- Issue date: 2008