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dc.contributor.authorLiu, YH
dc.contributor.authorBi, JX
dc.contributor.authorZeng, An-Ping
dc.contributor.authorYuan, JQ
dc.date.accessioned2008-04-14T09:17:54Z
dc.date.available2008-04-14T09:17:54Z
dc.date.issued2008-02-06
dc.identifier.citationA simple kinetic model for myeloma cell culture with consideration of lysine limitation. 2008:notBioprocess Biosyst Engen
dc.identifier.issn1615-7591
dc.identifier.pmid18253755
dc.identifier.doi10.1007/s00449-008-0204-x
dc.identifier.urihttp://hdl.handle.net/10033/23155
dc.description.abstractA simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.
dc.languageENG
dc.language.isonullen
dc.titleA simple kinetic model for myeloma cell culture with consideration of lysine limitation.
dc.typeArticleen
dc.contributor.departmentDepartment of Automation, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People’s Republic of China.en
dc.identifier.journalBioprocess and biosystems engineeringen
refterms.dateFOA2018-06-13T03:48:20Z
html.description.abstractA simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.


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