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dc.contributor.authorMeier, Karin
dc.contributor.authorMathieu, Eve-Lyne
dc.contributor.authorFinkernagel, Florian
dc.contributor.authorReuter, L Maximilian
dc.contributor.authorScharfe, Maren
dc.contributor.authorDoehlemann, Gunther
dc.contributor.authorJarek, Michael
dc.contributor.authorBrehm, Alexander
dc.date.accessioned2012-09-06T11:17:24Z
dc.date.available2012-09-06T11:17:24Z
dc.date.issued2012-05
dc.identifier.citationLINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes. 2012, 8 (5):e1002676 PLoS Genet.en_GB
dc.identifier.issn1553-7404
dc.identifier.pmid22570633
dc.identifier.doi10.1371/journal.pgen.1002676
dc.identifier.urihttp://hdl.handle.net/10033/241697
dc.description.abstractMutations in the l(3)mbt tumour suppressor result in overproliferation of Drosophila larval brains. Recently, the derepression of different gene classes in l(3)mbt mutants was shown to be causal for transformation. However, the molecular mechanisms of dL(3)mbt-mediated gene repression are not understood. Here, we identify LINT, the major dL(3)mbt complex of Drosophila. LINT has three core subunits-dL(3)mbt, dCoREST, and dLint-1-and is expressed in cell lines, embryos, and larval brain. Using genome-wide ChIP-Seq analysis, we show that dLint-1 binds close to the TSS of tumour-relevant target genes. Depletion of the LINT core subunits results in derepression of these genes. By contrast, histone deacetylase, histone methylase, and histone demethylase activities are not required to maintain repression. Our results support a direct role of LINT in the repression of brain tumour-relevant target genes by restricting promoter access.
dc.language.isoenen
dc.rightsArchived with thanks to PLoS geneticsen_GB
dc.titleLINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes.en
dc.typeArticleen
dc.contributor.departmentInstitut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Marburg, Germany.en_GB
dc.identifier.journalPLoS geneticsen_GB
refterms.dateFOA2018-06-13T01:21:53Z
html.description.abstractMutations in the l(3)mbt tumour suppressor result in overproliferation of Drosophila larval brains. Recently, the derepression of different gene classes in l(3)mbt mutants was shown to be causal for transformation. However, the molecular mechanisms of dL(3)mbt-mediated gene repression are not understood. Here, we identify LINT, the major dL(3)mbt complex of Drosophila. LINT has three core subunits-dL(3)mbt, dCoREST, and dLint-1-and is expressed in cell lines, embryos, and larval brain. Using genome-wide ChIP-Seq analysis, we show that dLint-1 binds close to the TSS of tumour-relevant target genes. Depletion of the LINT core subunits results in derepression of these genes. By contrast, histone deacetylase, histone methylase, and histone demethylase activities are not required to maintain repression. Our results support a direct role of LINT in the repression of brain tumour-relevant target genes by restricting promoter access.


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