Analysis of bacterial core communities in the central Baltic by comparative RNA-DNA-based fingerprinting provides links to structure-function relationships.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractUnderstanding structure-function links of microbial communities is a central theme of microbial ecology since its beginning. To this end, we studied the spatial variability of the bacterioplankton community structure and composition across the central Baltic Sea at four stations, which were up to 450 km apart and at a depth profile representative for the central part (Gotland Deep, 235 m). Bacterial community structure was followed by 16S ribosomal RNA (rRNA)- and 16S rRNA gene-based fingerprints using single-strand conformation polymorphism (SSCP) electrophoresis. Species composition was determined by sequence analysis of SSCP bands. High similarities of the bacterioplankton communities across several hundred kilometers were observed in the surface water using RNA- and DNA-based fingerprints. In these surface communities, the RNA- and DNA-based fingerprints resulted in very different pattern, presumably indicating large difference between the active members of the community as represented by RNA-based fingerprints and the present members represented by the DNA-based fingerprints. This large discrepancy changed gradually over depth, resulting in highly similar RNA- and DNA-based fingerprints in the anoxic part of the water column below 130 m depth. A conceivable mechanism explaining this high similarity could be the reduced oxidative stress in the anoxic zone. The stable communities on the surface and in the anoxic zone indicate the strong influence of the hydrography on the bacterioplankton community structure. Comparative analysis of RNA- and DNA-based community structure provided criteria for the identification of the core community, its key members and their links to biogeochemical functions.
CitationAnalysis of bacterial core communities in the central Baltic by comparative RNA-DNA-based fingerprinting provides links to structure-function relationships. 2012, 6 (1):195-212 ISME J
AffiliationDepartment of Vaccinology and Applied Microbiology, Helmholtz Centre of Infection Research (HZI), Braunschweig, Germany.
JournalThe ISME journal
The following license files are associated with this item:
- Bacterioneuston community structure in the southern Baltic sea and its dependence on meteorological conditions.
- Authors: Stolle C, Labrenz M, Meeske C, Jürgens K
- Issue date: 2011 Jun
- Spatial comparison of total vs. active bacterial populations by coupling genetic fingerprinting and clone library analyses in the NW Mediterranean Sea.
- Authors: Rodríguez-Blanco A, Ghiglione JF, Catala P, Casamayor EO, Lebaron P
- Issue date: 2009 Jan
- Specific detection, isolation, and characterization of selected, previously uncultured members of the freshwater bacterioplankton community.
- Authors: Gich F, Schubert K, Bruns A, Hoffelner H, Overmann J
- Issue date: 2005 Oct
- Composition and dynamics of bacterial communities of a drinking water supply system as assessed by RNA- and DNA-based 16S rRNA gene fingerprinting.
- Authors: Eichler S, Christen R, Höltje C, Westphal P, Bötel J, Brettar I, Mehling A, Höfle MG
- Issue date: 2006 Mar
- Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia.
- Authors: Hewson I, Fuhrman JA
- Issue date: 2004 Jun