Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe ATP-dependent chromatin remodeler dMi-2 can play both positive and negative roles in gene transcription. Recently, we have shown that dMi-2 is recruited to the hsp70 gene in a heat shock-dependent manner, and is required to achieve high transcript levels. Here, we use chromatin immunoprecipitation sequencing (ChIP-Seq) to identify other chromatin regions displaying increased dMi-2 binding upon heat shock and to characterize the distribution of dMi-2 over heat shock genes. We show that dMi-2 is recruited to the body of at least seven heat shock genes. Interestingly, dMi-2 binding extends several hundred base pairs beyond the polyadenylation site into the region where transcriptional termination occurs. We find that dMi-2 does not associate with the entire nucleosome-depleted hsp70 locus 87A. Rather, dMi-2 binding is restricted to transcribed regions. Our results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity.
CitationRecruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes. 2012, 40 (11):4879-91 Nucleic Acids Res.
AffiliationInstitute for Molecular Biology and Tumor Research, Philipps-University, Emil-Mannkopff-Strasse 2, 35037 Marburg, Germany.
JournalNucleic acids research
The following license files are associated with this item:
- Stress-induced PARP activation mediates recruitment of Drosophila Mi-2 to promote heat shock gene expression.
- Authors: Murawska M, Hassler M, Renkawitz-Pohl R, Ladurner A, Brehm A
- Issue date: 2011 Jul
- dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription.
- Authors: Murawska M, Kunert N, van Vugt J, Längst G, Kremmer E, Logie C, Brehm A
- Issue date: 2008 Apr
- dMi-2 chromatin binding and remodeling activities are regulated by dCK2 phosphorylation.
- Authors: Bouazoune K, Brehm A
- Issue date: 2005 Dec 23
- Rapid, transcription-independent loss of nucleosomes over a large chromatin domain at Hsp70 loci.
- Authors: Petesch SJ, Lis JT
- Issue date: 2008 Jul 11
- High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation.
- Authors: Andrulis ED, Guzmán E, Döring P, Werner J, Lis JT
- Issue date: 2000 Oct 15