Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes.
dc.contributor.author | Mathieu, Eve-Lyne | |
dc.contributor.author | Finkernagel, Florian | |
dc.contributor.author | Murawska, Magdalena | |
dc.contributor.author | Scharfe, Maren | |
dc.contributor.author | Jarek, Michael | |
dc.contributor.author | Brehm, Alexander | |
dc.date.accessioned | 2012-09-20T09:47:18Z | |
dc.date.available | 2012-09-20T09:47:18Z | |
dc.date.issued | 2012-06 | |
dc.identifier.citation | Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes. 2012, 40 (11):4879-91 Nucleic Acids Res. | en_GB |
dc.identifier.issn | 1362-4962 | |
dc.identifier.pmid | 22362736 | |
dc.identifier.doi | 10.1093/nar/gks178 | |
dc.identifier.uri | http://hdl.handle.net/10033/245183 | |
dc.description.abstract | The ATP-dependent chromatin remodeler dMi-2 can play both positive and negative roles in gene transcription. Recently, we have shown that dMi-2 is recruited to the hsp70 gene in a heat shock-dependent manner, and is required to achieve high transcript levels. Here, we use chromatin immunoprecipitation sequencing (ChIP-Seq) to identify other chromatin regions displaying increased dMi-2 binding upon heat shock and to characterize the distribution of dMi-2 over heat shock genes. We show that dMi-2 is recruited to the body of at least seven heat shock genes. Interestingly, dMi-2 binding extends several hundred base pairs beyond the polyadenylation site into the region where transcriptional termination occurs. We find that dMi-2 does not associate with the entire nucleosome-depleted hsp70 locus 87A. Rather, dMi-2 binding is restricted to transcribed regions. Our results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to Nucleic acids research | en_GB |
dc.subject.mesh | Adenosine Triphosphatases | en_GB |
dc.subject.mesh | Animals | en_GB |
dc.subject.mesh | Autoantigens | en_GB |
dc.subject.mesh | Cells, Cultured | en_GB |
dc.subject.mesh | Drosophila Proteins | en_GB |
dc.subject.mesh | Drosophila melanogaster | en_GB |
dc.subject.mesh | Genetic Loci | en_GB |
dc.subject.mesh | HSP70 Heat-Shock Proteins | en_GB |
dc.subject.mesh | Heat-Shock Proteins | en_GB |
dc.subject.mesh | Heat-Shock Response | en_GB |
dc.subject.mesh | Transcription, Genetic | en_GB |
dc.title | Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes. | en |
dc.type | Article | en |
dc.contributor.department | Institute for Molecular Biology and Tumor Research, Philipps-University, Emil-Mannkopff-Strasse 2, 35037 Marburg, Germany. | en_GB |
dc.identifier.journal | Nucleic acids research | en_GB |
refterms.dateFOA | 2018-06-13T00:37:24Z | |
html.description.abstract | The ATP-dependent chromatin remodeler dMi-2 can play both positive and negative roles in gene transcription. Recently, we have shown that dMi-2 is recruited to the hsp70 gene in a heat shock-dependent manner, and is required to achieve high transcript levels. Here, we use chromatin immunoprecipitation sequencing (ChIP-Seq) to identify other chromatin regions displaying increased dMi-2 binding upon heat shock and to characterize the distribution of dMi-2 over heat shock genes. We show that dMi-2 is recruited to the body of at least seven heat shock genes. Interestingly, dMi-2 binding extends several hundred base pairs beyond the polyadenylation site into the region where transcriptional termination occurs. We find that dMi-2 does not associate with the entire nucleosome-depleted hsp70 locus 87A. Rather, dMi-2 binding is restricted to transcribed regions. Our results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity. |