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dc.contributor.authorMathieu, Eve-Lyne
dc.contributor.authorFinkernagel, Florian
dc.contributor.authorMurawska, Magdalena
dc.contributor.authorScharfe, Maren
dc.contributor.authorJarek, Michael
dc.contributor.authorBrehm, Alexander
dc.date.accessioned2012-09-20T09:47:18Z
dc.date.available2012-09-20T09:47:18Z
dc.date.issued2012-06
dc.identifier.citationRecruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes. 2012, 40 (11):4879-91 Nucleic Acids Res.en_GB
dc.identifier.issn1362-4962
dc.identifier.pmid22362736
dc.identifier.doi10.1093/nar/gks178
dc.identifier.urihttp://hdl.handle.net/10033/245183
dc.description.abstractThe ATP-dependent chromatin remodeler dMi-2 can play both positive and negative roles in gene transcription. Recently, we have shown that dMi-2 is recruited to the hsp70 gene in a heat shock-dependent manner, and is required to achieve high transcript levels. Here, we use chromatin immunoprecipitation sequencing (ChIP-Seq) to identify other chromatin regions displaying increased dMi-2 binding upon heat shock and to characterize the distribution of dMi-2 over heat shock genes. We show that dMi-2 is recruited to the body of at least seven heat shock genes. Interestingly, dMi-2 binding extends several hundred base pairs beyond the polyadenylation site into the region where transcriptional termination occurs. We find that dMi-2 does not associate with the entire nucleosome-depleted hsp70 locus 87A. Rather, dMi-2 binding is restricted to transcribed regions. Our results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity.
dc.language.isoenen
dc.rightsArchived with thanks to Nucleic acids researchen_GB
dc.subject.meshAdenosine Triphosphatasesen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshAutoantigensen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshDrosophila Proteinsen_GB
dc.subject.meshDrosophila melanogasteren_GB
dc.subject.meshGenetic Locien_GB
dc.subject.meshHSP70 Heat-Shock Proteinsen_GB
dc.subject.meshHeat-Shock Proteinsen_GB
dc.subject.meshHeat-Shock Responseen_GB
dc.subject.meshTranscription, Geneticen_GB
dc.titleRecruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes.en
dc.typeArticleen
dc.contributor.departmentInstitute for Molecular Biology and Tumor Research, Philipps-University, Emil-Mannkopff-Strasse 2, 35037 Marburg, Germany.en_GB
dc.identifier.journalNucleic acids researchen_GB
refterms.dateFOA2018-06-13T00:37:24Z
html.description.abstractThe ATP-dependent chromatin remodeler dMi-2 can play both positive and negative roles in gene transcription. Recently, we have shown that dMi-2 is recruited to the hsp70 gene in a heat shock-dependent manner, and is required to achieve high transcript levels. Here, we use chromatin immunoprecipitation sequencing (ChIP-Seq) to identify other chromatin regions displaying increased dMi-2 binding upon heat shock and to characterize the distribution of dMi-2 over heat shock genes. We show that dMi-2 is recruited to the body of at least seven heat shock genes. Interestingly, dMi-2 binding extends several hundred base pairs beyond the polyadenylation site into the region where transcriptional termination occurs. We find that dMi-2 does not associate with the entire nucleosome-depleted hsp70 locus 87A. Rather, dMi-2 binding is restricted to transcribed regions. Our results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity.


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