A novel intranasal mouse model for mucosal colonization by Streptococcus suis serotype 2.
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Baums, Christoph Georg
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AbstractStreptococcus suis causes meningitis and various other diseases in pigs and humans. Healthy piglets carrying virulent Streptococcus suis strains on their mucosal surfaces are epidemiologically very important. The objective of this study was to establish an intranasal Streptococcus suis mouse model for invasion and colonization of the respiratory tract. CD1 mice were infected intranasally with a highly virulent Streptococcus suis serotype 2 strain under different conditions. Clinical, histological and bacteriological screenings revealed that invasion of host tissue occurred in the majority of mice only after predisposition with 12.5 µl 1 % acetic acid per nostril. Severe fibrinosuppurative or purulent necrotizing pneumonia associated with Streptococcus suis was a common manifestation. Furthermore, a novel model to study nasopharyngeal colonization was established by reducing the volume of 1 % acetic acid per nostril to 5 µl prior to Streptococcus suis application. This model mimics asymptomatic carriage in swine, as all mice carried Streptococcus suis on their respiratory mucosa at 7 days post-infection (p.i.) in moderate to high numbers without the development of pneumonia or any other invasive Streptococcus suis disease. This intranasal Streptococcus suis model was applied to investigate the function of suilysin (SLY) in colonization. Although an isogenic SLY mutant was isolated from the upper respiratory tract at a lower recovery rate than its wild-type parental strain at 14 days p.i., the differences were not significant and did not indicate severe attenuation in colonization. In conclusion, this work describes to the best of our knowledge the first intranasal mouse model to study colonization of the respiratory tract by a highly virulent Streptococcus suis pathotype.
CitationA novel intranasal mouse model for mucosal colonization by Streptococcus suis serotype 2. 2012, 61 (Pt 9):1311-8 J. Med. Microbiol.
Affiliation1Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany.
JournalJournal of medical microbiology
The following license files are associated with this item:
- Intranasal immunization with a live Streptococcus suis isogenic ofs mutant elicited suilysin-neutralization titers but failed to induce opsonizing antibodies and protection.
- Authors: Kock C, Beineke A, Seitz M, Ganter M, Waldmann KH, Valentin-Weigand P, Baums CG
- Issue date: 2009 Dec 15
- Role of suilysin in pathogenesis of Streptococcus suis capsular serotype 2.
- Authors: Lun S, Perez-Casal J, Connor W, Willson PJ
- Issue date: 2003 Jan
- Comparative evaluation of virulence and pathology of Streptococcus suis serotypes 2 and 9 in experimentally infected growers.
- Authors: Beineke A, Bennecke K, Neis C, Schröder C, Waldmann KH, Baumgärtner W, Valentin-Weigand P, Baums CG
- Issue date: 2008 Apr 30
- Distribution and genetic diversity of suilysin in Streptococcus suis isolated from different diseases of pigs and characterization of the genetic basis of suilysin absence.
- Authors: King SJ, Heath PJ, Luque I, Tarradas C, Dowson CG, Whatmore AM
- Issue date: 2001 Dec
- Role of capsule and suilysin in mucosal infection of complement-deficient mice with Streptococcus suis.
- Authors: Seitz M, Beineke A, Singpiel A, Willenborg J, Dutow P, Goethe R, Valentin-Weigand P, Klos A, Baums CG
- Issue date: 2014 Jun