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Authors
Chakraborty, AnirbanWang, Dongye
Ebright, Yon W
Korlann, You
Kortkhonjia, Ekaterine
Kim, Taiho
Chowdhury, Saikat
Wigneshweraraj, Sivaramesh
Irschik, Herbert
Jansen, Rolf
Nixon, B Tracy
Knight, Jennifer
Weiss, Shimon
Ebright, Richard H
Issue Date
2012-08-03
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Show full item recordAbstract
Using single-molecule fluorescence resonance energy transfer, we have defined bacterial RNA polymerase (RNAP) clamp conformation at each step in transcription initiation and elongation. We find that the clamp predominantly is open in free RNAP and early intermediates in transcription initiation but closes upon formation of a catalytically competent transcription initiation complex and remains closed during initial transcription and transcription elongation. We show that four RNAP inhibitors interfere with clamp opening. We propose that clamp opening allows DNA to be loaded into and unwound in the RNAP active-center cleft, that DNA loading and unwinding trigger clamp closure, and that clamp closure accounts for the high stability of initiation complexes and the high stability and processivity of elongation complexes.Citation
Opening and closing of the bacterial RNA polymerase clamp. 2012, 337 (6094):591-5 ScienceAffiliation
Howard Hughes Medical Institute, Waksman Institute, and Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA.Journal
Science (New York, N.Y.)PubMed ID
22859489Type
ArticleLanguage
enISSN
1095-9203ae974a485f413a2113503eed53cd6c53
10.1126/science.1218716
Scopus Count
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