Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells.
dc.contributor.author | van der Vlugt, Luciën E P M | |
dc.contributor.author | Labuda, Lucja A | |
dc.contributor.author | Ozir-Fazalalikhan, Arifa | |
dc.contributor.author | Lievers, Ellen | |
dc.contributor.author | Gloudemans, Anouk K | |
dc.contributor.author | Liu, Kit-Yeng | |
dc.contributor.author | Barr, Tom A | |
dc.contributor.author | Sparwasser, Tim | |
dc.contributor.author | Boon, Louis | |
dc.contributor.author | Ngoa, Ulysse Ateba | |
dc.contributor.author | Feugap, Eliane Ngoune | |
dc.contributor.author | Adegnika, Ayola A | |
dc.contributor.author | Kremsner, Peter G | |
dc.contributor.author | Gray, David | |
dc.contributor.author | Yazdanbakhsh, Maria | |
dc.contributor.author | Smits, Hermelijn H | |
dc.date.accessioned | 2012-12-07T15:29:08Z | |
dc.date.available | 2012-12-07T15:29:08Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells. 2012, 7 (2):e30883 PLoS ONE | en_GB |
dc.identifier.issn | 1932-6203 | |
dc.identifier.pmid | 22347409 | |
dc.identifier.doi | 10.1371/journal.pone.0030883 | |
dc.identifier.uri | http://hdl.handle.net/10033/254929 | |
dc.description.abstract | Chronic helminth infections, such as schistosomes, are negatively associated with allergic disorders. Here, using B cell IL-10-deficient mice, Schistosoma mansoni-mediated protection against experimental ovalbumin-induced allergic airway inflammation (AAI) was shown to be specifically dependent on IL-10-producing B cells. To study the organs involved, we transferred B cells from lungs, mesenteric lymph nodes or spleen of OVA-infected mice to recipient OVA-sensitized mice, and showed that both lung and splenic B cells reduced AAI, but only splenic B cells in an IL-10-dependent manner. Although splenic B cell protection was accompanied by elevated levels of pulmonary FoxP3(+) regulatory T cells, in vivo ablation of FoxP3(+) T cells only moderately restored AAI, indicating an important role for the direct suppressory effect of regulatory B cells. Splenic marginal zone CD1d(+) B cells proved to be the responsible splenic B cell subset as they produced high levels of IL-10 and induced FoxP3(+) T cells in vitro. Indeed, transfer of CD1d(+) MZ-depleted splenic B cells from infected mice restored AAI. Markedly, we found a similarly elevated population of CD1d(hi) B cells in peripheral blood of Schistosoma haematobium-infected Gabonese children compared to uninfected children and these cells produced elevated levels of IL-10. Importantly, the number of IL-10-producing CD1d(hi) B cells was reduced after anti-schistosome treatment. This study points out that in both mice and men schistosomes have the capacity to drive the development of IL-10-producing regulatory CD1d(hi) B cells and furthermore, these are instrumental in reducing experimental allergic inflammation in mice. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to PloS one | en_GB |
dc.subject.mesh | Animals | en_GB |
dc.subject.mesh | Antigens, CD1d | en_GB |
dc.subject.mesh | B-Lymphocytes | en_GB |
dc.subject.mesh | Child | en_GB |
dc.subject.mesh | Gabon | en_GB |
dc.subject.mesh | Helminths | en_GB |
dc.subject.mesh | Humans | en_GB |
dc.subject.mesh | Hypersensitivity | en_GB |
dc.subject.mesh | Inflammation | en_GB |
dc.subject.mesh | Interleukin-10 | en_GB |
dc.subject.mesh | Mice | en_GB |
dc.subject.mesh | Mice, Knockout | en_GB |
dc.subject.mesh | Schistosoma | en_GB |
dc.subject.mesh | T-Lymphocytes, Regulatory | en_GB |
dc.title | Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells. | en |
dc.type | Article | en |
dc.contributor.department | Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands. | en_GB |
dc.identifier.journal | PloS one | en_GB |
refterms.dateFOA | 2018-06-13T15:48:57Z | |
html.description.abstract | Chronic helminth infections, such as schistosomes, are negatively associated with allergic disorders. Here, using B cell IL-10-deficient mice, Schistosoma mansoni-mediated protection against experimental ovalbumin-induced allergic airway inflammation (AAI) was shown to be specifically dependent on IL-10-producing B cells. To study the organs involved, we transferred B cells from lungs, mesenteric lymph nodes or spleen of OVA-infected mice to recipient OVA-sensitized mice, and showed that both lung and splenic B cells reduced AAI, but only splenic B cells in an IL-10-dependent manner. Although splenic B cell protection was accompanied by elevated levels of pulmonary FoxP3(+) regulatory T cells, in vivo ablation of FoxP3(+) T cells only moderately restored AAI, indicating an important role for the direct suppressory effect of regulatory B cells. Splenic marginal zone CD1d(+) B cells proved to be the responsible splenic B cell subset as they produced high levels of IL-10 and induced FoxP3(+) T cells in vitro. Indeed, transfer of CD1d(+) MZ-depleted splenic B cells from infected mice restored AAI. Markedly, we found a similarly elevated population of CD1d(hi) B cells in peripheral blood of Schistosoma haematobium-infected Gabonese children compared to uninfected children and these cells produced elevated levels of IL-10. Importantly, the number of IL-10-producing CD1d(hi) B cells was reduced after anti-schistosome treatment. This study points out that in both mice and men schistosomes have the capacity to drive the development of IL-10-producing regulatory CD1d(hi) B cells and furthermore, these are instrumental in reducing experimental allergic inflammation in mice. |
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