High efficient adenoviral-mediated VEGF and Ang-1 gene delivery into osteogenically differentiated human mesenchymal stem cells.

Klöpper, Jonas; Lindenmaier, Werner; Fiedler, Ulrike; Mehlhorn, Alexander; Stark, G Björn; Finkenzeller, Günter

dc.contributor.author	Klöpper, Jonas
dc.contributor.author	Lindenmaier, Werner
dc.contributor.author	Fiedler, Ulrike
dc.contributor.author	Mehlhorn, Alexander
dc.contributor.author	Stark, G Björn
dc.contributor.author	Finkenzeller, Günter
dc.date.accessioned	2008-05-13T10:42:15Z
dc.date.available	2008-05-13T10:42:15Z
dc.date.issued	2008-01
dc.identifier.citation	High efficient adenoviral-mediated VEGF and Ang-1 gene delivery into osteogenically differentiated human mesenchymal stem cells. 2008, 75 (1):83-90 Microvasc. Res.	en
dc.identifier.issn	0026-2862
dc.identifier.pmid	17603084
dc.identifier.doi	10.1016/j.mvr.2007.04.010
dc.identifier.uri	http://hdl.handle.net/10033/25714
dc.description.abstract	Survival of ex vivo constructed tissues after transplantation is limited by insufficient oxygen and nutrient supply. Therefore, strategies aiming at improvement of neovascularization of engineered tissues are a key issue in tissue engineering applications. This in vitro study aimed at exploring the usability of osteogenically differentiated human mesenchymal stem cells (MSCs) as carriers of the angiogenic growth factor genes vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) for therapeutic angiogenesis in bone tissue engineering. The ex vivo adenoviral vector mediated transduction into osteogenically differentiated MSCs revealed a highly efficient and long lasting expression of the transgenes. Biological activity of VEGF and Ang-1 secreted from transduced cells was confirmed by analyzing the sprouting, proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) in response to conditioned medium obtained from transduced cells. The transduced osteogenically differentiated MSCs described in this report may be suitable for inducing neovascularization in bone tissue engineering applications.
dc.language.iso	en	en
dc.subject.mesh	Adenoviridae	en
dc.subject.mesh	Adult Stem Cells	en
dc.subject.mesh	Angiopoietin-1	en
dc.subject.mesh	Apoptosis	en
dc.subject.mesh	Cell Differentiation	en
dc.subject.mesh	Cell Proliferation	en
dc.subject.mesh	Cells, Cultured	en
dc.subject.mesh	Culture Media, Conditioned	en
dc.subject.mesh	Endothelial Cells	en
dc.subject.mesh	Genetic Vectors	en
dc.subject.mesh	Humans	en
dc.subject.mesh	Mesenchymal Stem Cells	en
dc.subject.mesh	Neovascularization, Physiologic	en
dc.subject.mesh	Osteoblasts	en
dc.subject.mesh	Osteogenesis	en
dc.subject.mesh	Spheroids, Cellular	en
dc.subject.mesh	Time Factors	en
dc.subject.mesh	Tissue Engineering	en
dc.subject.mesh	Transduction, Genetic	en
dc.subject.mesh	Vascular Endothelial Growth Factor A	en
dc.title	High efficient adenoviral-mediated VEGF and Ang-1 gene delivery into osteogenically differentiated human mesenchymal stem cells.	en
dc.type	Article	en
dc.contributor.department	Department of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, Germany.	en
dc.identifier.journal	Microvascular research	en
refterms.dateFOA	2018-06-13T00:30:21Z
html.description.abstract	Survival of ex vivo constructed tissues after transplantation is limited by insufficient oxygen and nutrient supply. Therefore, strategies aiming at improvement of neovascularization of engineered tissues are a key issue in tissue engineering applications. This in vitro study aimed at exploring the usability of osteogenically differentiated human mesenchymal stem cells (MSCs) as carriers of the angiogenic growth factor genes vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) for therapeutic angiogenesis in bone tissue engineering. The ex vivo adenoviral vector mediated transduction into osteogenically differentiated MSCs revealed a highly efficient and long lasting expression of the transgenes. Biological activity of VEGF and Ang-1 secreted from transduced cells was confirmed by analyzing the sprouting, proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) in response to conditioned medium obtained from transduced cells. The transduced osteogenically differentiated MSCs described in this report may be suitable for inducing neovascularization in bone tissue engineering applications.