Autoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Yip, Cindy Ying Yin
Levesque, Celine M
Cvitkovitch, Dennis G
MetadataShow full item record
AbstractAutoinducer 2 (AI-2) is the only species-nonspecific autoinducer known in bacteria and is produced by both gram-negative and gram-positive organisms. Consequently, it is proposed to function as a universal quorum-sensing signal for interaction between bacterial species. AI-2 is produced as the by-product of a metabolic transformation carried out by the LuxS enzyme. To separate the metabolic function of the LuxS enzyme from the signaling role of AI-2, we carried out a global transcriptome analysis of a luxS null mutant culture of Streptococcus mutans UA159, an important cariogenic bacterium and a crucial component of the dental plaque biofilm community, in comparison to a luxS null mutant culture supplemented with chemically pure 4,5-dihydroxy-2,3-pentanedione, the precursor of AI-2. The data revealed fundamental changes in gene expression affecting 585 genes (30% of the genome) which could not be restored by the signal molecule AI-2 and are therefore not caused by quorum sensing but by lack of the transformation carried out by the LuxS enzyme in the activated methyl cycle. All functional classes of enzymes were affected, including genes known to be important for biofilm formation, bacteriocin synthesis, competence, and acid tolerance. At the same time, 59 genes were identified whose transcription clearly responded to the addition of AI-2. Some of them were related to protein synthesis, stress, and cell division. Three membrane transport proteins were upregulated which are not related to any of the known AI-2 transporters. Three transcription factors were identified whose transcription was stimulated repeatedly by AI-2 addition during growth. Finally, a global regulatory protein, the delta subunit of the RNA polymerase (rpoE), was induced 147-fold by AI-2, representing the largest differential gene expression observed. The data show that many phenotypes related to the luxS mutation cannot be ascribed to quorum sensing and have identified for the first time regulatory proteins potentially mediating AI-2-based signaling in gram-positive bacteria.
CitationAutoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation. 2008, 190 (1):401-15 J. Bacteriol.
AffiliationHelmholtz-Center for Infection Research, Division of Cell Biology, Inhoffenstr. 7, D-38124 Braunschweig, Germany.
JournalJournal of bacteriology
The following license files are associated with this item:
- Streptococcus gordonii LuxS/autoinducer-2 quorum-sensing system modulates the dual-species biofilm formation with Streptococcus mutans.
- Authors: Wang X, Li X, Ling J
- Issue date: 2017 Jul
- Analysis of AI-2/LuxS-dependent transcription in Campylobacter jejuni strain 81-176.
- Authors: He Y, Frye JG, Strobaugh TP, Chen CY
- Issue date: 2008 Aug
- Mutation of luxS affects biofilm formation in Streptococcus mutans.
- Authors: Merritt J, Qi F, Goodman SD, Anderson MH, Shi W
- Issue date: 2003 Apr
- Comparative transcriptomics analysis of Streptococcus mutans with disruption of LuxS/AI-2 quorum sensing and recovery of methyl cycle.
- Authors: Yuan K, Hou L, Jin Q, Niu C, Mao M, Wang R, Huang Z
- Issue date: 2021 Jul
- The LuxS/AI-2 Quorum-Sensing System of <i>Streptococcus pneumoniae</i> Is Required to Cause Disease, and to Regulate Virulence- and Metabolism-Related Genes in a Rat Model of Middle Ear Infection.
- Authors: Yadav MK, Vidal JE, Go YY, Kim SH, Chae SW, Song JJ
- Issue date: 2018