Specific acquisition of functional CD59 but not CD46 or CD55 by hepatitis C virus.
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Authors
Ejaz, AsimSteinmann, Eike
Bánki, Zoltán
Anggakusuma
Khalid, Sana
Lengauer, Susanne
Wilhelm, Corinne
Zoller, Heinz
Schloegl, Anna
Steinmann, Joerg
Grabski, Elena
Kleines, Michael
Pietschmann, Thomas
Stoiber, Heribert
Issue Date
2012
Metadata
Show full item recordAbstract
Viruses of different families encode for regulators of the complement system (RCAs) or acquire such RCAs from the host to get protection against complement-mediated lysis (CML). As hepatitis C virus (HCV) shares no genetic similarity to any known RCA and is detectable at high titers in sera of infected individuals, we investigated whether HCV has adapted host-derived RCAs to resist CML. Here we report that HCV selectively incorporates CD59 while neither CD55, nor CD46 are associated with the virus. The presence of CD59 was shown by capture assays using patient- and cell culture-derived HCV isolates. Association of CD59 with HCV was further confirmed by Western blot analysis using purified viral supernatants from infected Huh 7.5 cells. HCV captured by antibodies specific for CD59 remained infectious for Huh 7.5 cells. In addition, blocking of CD59 in the presence of active complement reduced the titer of HCV most likely due to CML. HCV produced in CD59 knock-down cells were more significantly susceptible to CML compared to wild type virus, but neither replication, assembly nor infectivity of the virus seemed to be impaired in the absence of CD59. In summary our data indicate that HCV incorporates selectively CD59 in its envelope to gain resistance to CML in serum of infected individuals.Citation
Specific acquisition of functional CD59 but not CD46 or CD55 by hepatitis C virus. 2012, 7 (9):e45770 PLoS ONEAffiliation
Institute of Virology, Innsbruck Medical University, Innsbruck, Austria.Journal
PloS onePubMed ID
23049856Type
ArticleLanguage
enISSN
1932-6203ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0045770
Scopus Count
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