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dc.contributor.authorDandekar, Prajakta
dc.contributor.authorJain, Ratnesh
dc.contributor.authorKeil, Manuel
dc.contributor.authorLoretz, Brigitta
dc.contributor.authorMuijs, Leon
dc.contributor.authorSchneider, Marc
dc.contributor.authorAuerbach, Dagmar
dc.contributor.authorJung, Gregor
dc.contributor.authorLehr, Claus-Michael
dc.contributor.authorWenz, Gerhard
dc.date.accessioned2013-01-22T15:26:29Zen
dc.date.available2013-01-22T15:26:29Zen
dc.date.issued2012-12-28en
dc.identifier.citationCellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes. 2012, 164 (3):387-93 J Control Releaseen_GB
dc.identifier.issn1873-4995en
dc.identifier.pmid22789529en
dc.identifier.doi10.1016/j.jconrel.2012.06.040en
dc.identifier.urihttp://hdl.handle.net/10033/266513en
dc.description.abstractCationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest.
dc.language.isoenen
dc.rightsArchived with thanks to Journal of controlled release : official journal of the Controlled Release Societyen_GB
dc.titleCellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes.en
dc.typeArticleen
dc.contributor.departmentDepartment of Drug Delivery, Helmholtz-Institute for Pharmaceutical Research Saarland, Helmholtz-Center for Infection Research (HZI), Saarland University, D-66123 Saarbrücken, Germany.en_GB
dc.identifier.journalJournal of controlled release : official journal of the Controlled Release Societyen_GB
refterms.dateFOA2018-06-12T23:12:10Z
html.description.abstractCationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest.


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