Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes.
dc.contributor.author | Dandekar, Prajakta | |
dc.contributor.author | Jain, Ratnesh | |
dc.contributor.author | Keil, Manuel | |
dc.contributor.author | Loretz, Brigitta | |
dc.contributor.author | Muijs, Leon | |
dc.contributor.author | Schneider, Marc | |
dc.contributor.author | Auerbach, Dagmar | |
dc.contributor.author | Jung, Gregor | |
dc.contributor.author | Lehr, Claus-Michael | |
dc.contributor.author | Wenz, Gerhard | |
dc.date.accessioned | 2013-01-22T15:26:29Z | en |
dc.date.available | 2013-01-22T15:26:29Z | en |
dc.date.issued | 2012-12-28 | en |
dc.identifier.citation | Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes. 2012, 164 (3):387-93 J Control Release | en_GB |
dc.identifier.issn | 1873-4995 | en |
dc.identifier.pmid | 22789529 | en |
dc.identifier.doi | 10.1016/j.jconrel.2012.06.040 | en |
dc.identifier.uri | http://hdl.handle.net/10033/266513 | en |
dc.description.abstract | Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to Journal of controlled release : official journal of the Controlled Release Society | en_GB |
dc.title | Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes. | en |
dc.type | Article | en |
dc.contributor.department | Department of Drug Delivery, Helmholtz-Institute for Pharmaceutical Research Saarland, Helmholtz-Center for Infection Research (HZI), Saarland University, D-66123 Saarbrücken, Germany. | en_GB |
dc.identifier.journal | Journal of controlled release : official journal of the Controlled Release Society | en_GB |
refterms.dateFOA | 2018-06-12T23:12:10Z | |
html.description.abstract | Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest. |