Variability in the distribution of genes encoding virulence factors and putative extracellular proteins of Streptococcus pyogenes in India, a region with high streptococcal disease burden, and implication for development of a regional multisubunit vaccine.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
McMillan, David J
Nitsche Schmitz, D Patric
Chhatwal, Gursharan S
MetadataShow full item record
AbstractStreptococcus pyogenes causes a wide variety of human diseases and is a significant cause of morbidity and mortality. Attempts to develop a vaccine were hampered by the genetic diversity of S. pyogenes across different regions of the world. This study sought to identify streptococcal antigens suitable for a region-specific vaccine in India. We used a two-step approach, first performing epidemiological analysis to identify the conserved antigens among Indian isolates. The second step consisted of validating the identified antigens by serological analysis. The 201 streptococcal clinical isolates from India used in this study represented 69 different emm types, with emm12 being the most prevalent. Virulence profiling of the North and South Indian S. pyogenes isolates with a custom-designed streptococcal virulence microarray identified seven conserved putative vaccine candidates. Collagen-like surface protein (SCI), putative secreted 5'-nucleotidase (PSNT), and C5a peptidase were found in 100% of the isolates, while R28, a putative surface antigen (PSA), and a hypothetical protein (HYP) were found in 90% of the isolates. A fibronectin binding protein, SfbI, was present in only 78% of the isolates. In order to validate the identified potential vaccine candidates, 185 serum samples obtained from patients with different clinical manifestations were tested for antibodies. Irrespective of clinical manifestations, serum samples showed high antibody titers to all proteins except for SCI and R28. Thus, the data indicate that PSNT, C5a peptidase, PSA, HYP, and SfbI are promising candidates for a region-specific streptococcal vaccine for the different parts of India.
CitationVariability in the distribution of genes encoding virulence factors and putative extracellular proteins of Streptococcus pyogenes in India, a region with high streptococcal disease burden, and implication for development of a regional multisubunit vaccine. 2012, 19 (11):1818-25 Clin. Vaccine Immunol.
AffiliationDepartment of Medical Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
The following license files are associated with this item:
- Comparative analysis of emm type pattern of Group A Streptococcus throat and skin isolates from India and their association with closely related SIC, a streptococcal virulence factor.
- Authors: Sagar V, Kumar R, Ganguly NK, Chakraborti A
- Issue date: 2008 Sep 16
- Population and Whole Genome Sequence Based Characterization of Invasive Group A Streptococci Recovered in the United States during 2015.
- Authors: Chochua S, Metcalf BJ, Li Z, Rivers J, Mathis S, Jackson D, Gertz RE Jr, Srinivasan V, Lynfield R, Van Beneden C, McGee L, Beall B
- Issue date: 2017 Sep 19
- Molecular heterogeneity among north Indian isolates of Group A Streptococcus.
- Authors: Sagar V, Bakshi DK, Nandi S, Ganguly NK, Kumar R, Chakraborti A
- Issue date: 2004
- Molecular biology of Group A <i>Streptococcus</i> and its implications in vaccine strategies.
- Authors: Brahmadathan NK
- Issue date: 2017 Apr-Jun
- Clinical and microbiologic characteristics of invasive Streptococcus pyogenes infections in north and south India.
- Authors: Haggar A, Nerlich A, Kumar R, Abraham VJ, Brahmadathan KN, Ray P, Dhanda V, Joshua JM, Mehra N, Bergmann R, Chhatwal GS, Norrby-Teglund A
- Issue date: 2012 May