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dc.contributor.authorWöhl-Bruhn, Stefanie
dc.contributor.authorBadar, Muhammad
dc.contributor.authorBertz, Andreas
dc.contributor.authorTiersch, Brigitte
dc.contributor.authorKoetz, Joachim
dc.contributor.authorMenzel, Henning
dc.contributor.authorMueller, Peter P
dc.contributor.authorBunjes, Heike
dc.date.accessioned2013-02-11T14:43:02Z
dc.date.available2013-02-11T14:43:02Z
dc.date.issued2012-08-20
dc.identifier.citationComparison of in vitro and in vivo protein release from hydrogel systems. 2012, 162 (1):127-33 J Control Releaseen_GB
dc.identifier.issn1873-4995
dc.identifier.pmid22687287
dc.identifier.doi10.1016/j.jconrel.2012.05.049
dc.identifier.urihttp://hdl.handle.net/10033/269012
dc.description.abstractHydrogel systems based on hydroxyethyl starch-polyethylene glycol methacrylate (HES-P(EG)(6)MA) or hydroxyethyl starch methacrylate (HES-MA) were used to assess the protein release behavior. Here, we analyzed the in vitro release of FITC-anti-human antibodies incorporated in either HES-P(EG)(6)MA or HES-MA hydrogel delivery systems in PBS or human serum. In addition, hydrogel disks and microparticles prepared from the two polymers were subcutaneously implanted in BALB/c mice. The in vivo release of FITC-IgG was non-invasively monitored by an in vivo imaging system (IVIS 200) over a time period of up to 3 months. The imaging system allowed to asses individual animals over time, therefore only a small number of animals was required to obtain high quality data. The reduction in fluorescence intensity at the site of administration was compared to in vitro release profiles. These investigations demonstrated a sustained release from HES-MA hydrogel disks compared to rapidly degrading HES-P(EG)(6)MA disks and microparticles. The sustained release from HES-MA disks could be further optimized by using increased polymer concentrations. Human serum as in vitro release medium reflected better the in vivo release from HES-P(EG)(6)MA systems than PBS, suggesting that the presence of organic substances like proteins or lipids may play a significant role for the release kinetics.
dc.language.isoenen
dc.rightsArchived with thanks to Journal of controlled release : official journal of the Controlled Release Societyen_GB
dc.subject.meshAbsorbable Implantsen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshDrug Delivery Systemsen_GB
dc.subject.meshFluorescein-5-isothiocyanateen_GB
dc.subject.meshGoatsen_GB
dc.subject.meshHetastarchen_GB
dc.subject.meshHumansen_GB
dc.subject.meshHydrogelen_GB
dc.subject.meshImmunoglobulin Gen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMethacrylatesen_GB
dc.subject.meshMiceen_GB
dc.subject.meshMice, Inbred BALB Cen_GB
dc.subject.meshPolyethylene Glycolsen_GB
dc.titleComparison of in vitro and in vivo protein release from hydrogel systems.en
dc.typeArticleen
dc.contributor.departmentTechnische Universität Braunschweig, Institute of Pharmaceutical Technology, Mendelssohnstraße 1, 38106 Braunschweig, Germany.en_GB
dc.identifier.journalJournal of controlled release : official journal of the Controlled Release Societyen_GB
refterms.dateFOA2018-06-12T23:38:19Z
html.description.abstractHydrogel systems based on hydroxyethyl starch-polyethylene glycol methacrylate (HES-P(EG)(6)MA) or hydroxyethyl starch methacrylate (HES-MA) were used to assess the protein release behavior. Here, we analyzed the in vitro release of FITC-anti-human antibodies incorporated in either HES-P(EG)(6)MA or HES-MA hydrogel delivery systems in PBS or human serum. In addition, hydrogel disks and microparticles prepared from the two polymers were subcutaneously implanted in BALB/c mice. The in vivo release of FITC-IgG was non-invasively monitored by an in vivo imaging system (IVIS 200) over a time period of up to 3 months. The imaging system allowed to asses individual animals over time, therefore only a small number of animals was required to obtain high quality data. The reduction in fluorescence intensity at the site of administration was compared to in vitro release profiles. These investigations demonstrated a sustained release from HES-MA hydrogel disks compared to rapidly degrading HES-P(EG)(6)MA disks and microparticles. The sustained release from HES-MA disks could be further optimized by using increased polymer concentrations. Human serum as in vitro release medium reflected better the in vivo release from HES-P(EG)(6)MA systems than PBS, suggesting that the presence of organic substances like proteins or lipids may play a significant role for the release kinetics.


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