Metaproteogenomic insights beyond bacterial response to naphthalene exposure and bio-stimulation.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Peláez, Ana Isabel
Del Pozo, Mercedes V
Vieites, José María
von Bergen, Martin
Gallego, José Luis R
Pieper, Dietmar H
MetadataShow full item record
AbstractMicrobial metabolism in aromatic-contaminated environments has important ecological implications, and obtaining a complete understanding of this process remains a relevant goal. To understand the roles of biodiversity and aromatic-mediated genetic and metabolic rearrangements, we conducted 'OMIC' investigations in an anthropogenically influenced and polyaromatic hydrocarbon (PAH)-contaminated soil with (Nbs) or without (N) bio-stimulation with calcium ammonia nitrate, NH(4)NO(3) and KH(2)PO(4) and the commercial surfactant Iveysol, plus two naphthalene-enriched communities derived from both soils (CN2 and CN1, respectively). Using a metagenomic approach, a total of 52, 53, 14 and 12 distinct species (according to operational phylogenetic units (OPU) in our work equivalent to taxonomic species) were identified in the N, Nbs, CN1 and CN2 communities, respectively. Approximately 10 out of 95 distinct species and 238 out of 3293 clusters of orthologous groups (COGs) protein families identified were clearly stimulated under the assayed conditions, whereas only two species and 1465 COGs conformed to the common set in all of the mesocosms. Results indicated distinct biodegradation capabilities for the utilisation of potential growth-supporting aromatics, which results in bio-stimulated communities being extremely fit to naphthalene utilisation and non-stimulated communities exhibiting a greater metabolic window than previously predicted. On the basis of comparing protein expression profiles and metagenome data sets, inter-alia interactions among members were hypothesised. The utilisation of curated databases is discussed and used for first time to reconstruct 'presumptive' degradation networks for complex microbial communities.
CitationMetaproteogenomic insights beyond bacterial response to naphthalene exposure and bio-stimulation. 2013, 7 (1):122-36 ISME J
AffiliationDepartment of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain.
JournalThe ISME journal
The following license files are associated with this item:
- Context-specific metabolic network reconstruction of a naphthalene-degrading bacterial community guided by metaproteomic data.
- Authors: Tobalina L, Bargiela R, Pey J, Herbst FA, Lores I, Rojo D, Barbas C, Peláez AI, Sánchez J, von Bergen M, Seifert J, Ferrer M, Planes FJ
- Issue date: 2015 Jun 1
- Resilience and Assemblage of Soil Microbiome in Response to Chemical Contamination Combined with Plant Growth.
- Authors: Jiao S, Chen W, Wei G
- Issue date: 2019 Mar 15
- Metagenomic analysis of microbial community and function involved in cd-contaminated soil.
- Authors: Feng G, Xie T, Wang X, Bai J, Tang L, Zhao H, Wei W, Wang M, Zhao Y
- Issue date: 2018 Feb 13
- Identification of bacteria utilizing biphenyl, benzoate, and naphthalene in long-term contaminated soil.
- Authors: Uhlik O, Wald J, Strejcek M, Musilova L, Ridl J, Hroudova M, Vlcek C, Cardenas E, Mackova M, Macek T
- Issue date: 2012
- Stable-isotope probing of the polycyclic aromatic hydrocarbon-degrading bacterial guild in a contaminated soil.
- Authors: Jones MD, Crandell DW, Singleton DR, Aitken MD
- Issue date: 2011 Oct