Metaproteogenomic insights beyond bacterial response to naphthalene exposure and bio-stimulation.
Name:
Guazzaroni et al_final.pdf
Size:
2.891Mb
Format:
PDF
Description:
OPen Access article
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Guazzaroni, María-EugeniaHerbst, Florian-Alexander
Lores, Iván
Tamames, Javier
Peláez, Ana Isabel
López-Cortés, Nieves
Alcaide, María
Del Pozo, Mercedes V
Vieites, José María
von Bergen, Martin
Gallego, José Luis R
Bargiela, Rafael
López-López, Arantxa
Pieper, Dietmar H
Rosselló-Móra, Ramón
Sánchez, Jesús
Seifert, Jana
Ferrer, Manuel
Issue Date
2013-01
Metadata
Show full item recordAbstract
Microbial metabolism in aromatic-contaminated environments has important ecological implications, and obtaining a complete understanding of this process remains a relevant goal. To understand the roles of biodiversity and aromatic-mediated genetic and metabolic rearrangements, we conducted 'OMIC' investigations in an anthropogenically influenced and polyaromatic hydrocarbon (PAH)-contaminated soil with (Nbs) or without (N) bio-stimulation with calcium ammonia nitrate, NH(4)NO(3) and KH(2)PO(4) and the commercial surfactant Iveysol, plus two naphthalene-enriched communities derived from both soils (CN2 and CN1, respectively). Using a metagenomic approach, a total of 52, 53, 14 and 12 distinct species (according to operational phylogenetic units (OPU) in our work equivalent to taxonomic species) were identified in the N, Nbs, CN1 and CN2 communities, respectively. Approximately 10 out of 95 distinct species and 238 out of 3293 clusters of orthologous groups (COGs) protein families identified were clearly stimulated under the assayed conditions, whereas only two species and 1465 COGs conformed to the common set in all of the mesocosms. Results indicated distinct biodegradation capabilities for the utilisation of potential growth-supporting aromatics, which results in bio-stimulated communities being extremely fit to naphthalene utilisation and non-stimulated communities exhibiting a greater metabolic window than previously predicted. On the basis of comparing protein expression profiles and metagenome data sets, inter-alia interactions among members were hypothesised. The utilisation of curated databases is discussed and used for first time to reconstruct 'presumptive' degradation networks for complex microbial communities.Citation
Metaproteogenomic insights beyond bacterial response to naphthalene exposure and bio-stimulation. 2013, 7 (1):122-36 ISME JAffiliation
Department of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain.Journal
The ISME journalPubMed ID
22832345Type
ArticleLanguage
enISSN
1751-7370ae974a485f413a2113503eed53cd6c53
10.1038/ismej.2012.82
Scopus Count
The following license files are associated with this item:
Related articles
- Context-specific metabolic network reconstruction of a naphthalene-degrading bacterial community guided by metaproteomic data.
- Authors: Tobalina L, Bargiela R, Pey J, Herbst FA, Lores I, Rojo D, Barbas C, Peláez AI, Sánchez J, von Bergen M, Seifert J, Ferrer M, Planes FJ
- Issue date: 2015 Jun 1
- Resilience and Assemblage of Soil Microbiome in Response to Chemical Contamination Combined with Plant Growth.
- Authors: Jiao S, Chen W, Wei G
- Issue date: 2019 Mar 15
- Metagenomic analysis of microbial community and function involved in cd-contaminated soil.
- Authors: Feng G, Xie T, Wang X, Bai J, Tang L, Zhao H, Wei W, Wang M, Zhao Y
- Issue date: 2018 Feb 13
- Identification of bacteria utilizing biphenyl, benzoate, and naphthalene in long-term contaminated soil.
- Authors: Uhlik O, Wald J, Strejcek M, Musilova L, Ridl J, Hroudova M, Vlcek C, Cardenas E, Mackova M, Macek T
- Issue date: 2012
- Phylogenetic and Functional Diversity of Total (DNA) and Expressed (RNA) Bacterial Communities in Urban Green Infrastructure Bioswale Soils.
- Authors: Gill AS, Lee A, McGuire KL
- Issue date: 2017 Aug 15