Growth Medium-Dependent Glycine Incorporation into the Peptidoglycan of Caulobacter crescentus.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsTakacs, Constantin N
Cabeen, Matthew T
Bui, Nhat Khai
MetadataShow full item record
AbstractThe peptidoglycan (PG) is a macromolecular component of the bacterial cell wall that maintains the shape and integrity of the cell. The PG of , unlike that of many other Gram-negative bacteria, has repeatedly been shown to contain significant amounts of glycine. This compositional peculiarity has been deemed an intrinsic characteristic of this species. By performing a comprehensive qualitative and quantitative analysis of the PG by high-performance liquid chromatography (HPLC) and mass spectrometry (MS), we show here that glycine incorporation into the PG depends on the presence of exogenous glycine in the growth medium. High levels of glycine were detected at the fifth position of the peptide side chains of PG isolated from cells grown in the complex laboratory medium PYE or in defined medium (M2G) supplemented with casamino acids or glycine alone. In contrast, glycine incorporation was undetectable when cells were grown in M2G medium lacking glycine. Remarkably, glycine incorporation into peptidoglycan occurred even in the presence of low millimolar to sub-millimolar concentrations of free glycine. High glycine content in the PG had no obvious effects on growth rates, mode of PG incorporation or cell morphology. Hence, the PG is able to retain its physiological functions in cell growth and morphogenesis despite significant alterations in its composition, in what we deem to be unprecedented plasticity.
CitationGrowth Medium-Dependent Glycine Incorporation into the Peptidoglycan of Caulobacter crescentus. 2013, 8 (2):e57579 PLoS ONE
AffiliationDepartment of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, United States of America.
The following license files are associated with this item:
- Autolysis of Caulobacter crescentus grown in the presence of glycine.
- Authors: Markiewicz Z, Kwiatkowski Z
- Issue date: 1985
- A protein critical for cell constriction in the Gram-negative bacterium Caulobacter crescentus localizes at the division site through its peptidoglycan-binding LysM domains.
- Authors: Poggio S, Takacs CN, Vollmer W, Jacobs-Wagner C
- Issue date: 2010 Jul 1
- Loss of Bacterial Cell Pole Stabilization in Caulobacter crescentus Sensitizes to Outer Membrane Stress and Peptidoglycan-Directed Antibiotics.
- Authors: Vallet SU, Hansen LH, Bistrup FC, Laursen SA, Chapalay JB, Chambon M, Turcatti G, Viollier PH, Kirkpatrick CL
- Issue date: 2020 May 5
- Processivity of peptidoglycan synthesis provides a built-in mechanism for the robustness of straight-rod cell morphology.
- Authors: Sliusarenko O, Cabeen MT, Wolgemuth CW, Jacobs-Wagner C, Emonet T
- Issue date: 2010 Jun 1
- Transcriptional profiling of Caulobacter crescentus during growth on complex and minimal media.
- Authors: Hottes AK, Meewan M, Yang D, Arana N, Romero P, McAdams HH, Stephens C
- Issue date: 2004 Mar