Sensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Sundberg, John P
von Wasielewski, Reinhard
de Buhr, Maike F
Janus, Lydia M
Ukena, Sya N
MetadataShow full item record
AbstractEscherichia coli Nissle 1917 (EcN) is a well-characterized probiotic bacterium. Although genomic comparisons of EcN with the uropathogenic E. coli strain CFT073 revealed high degrees of similarity, EcN is generally considered a non-pathogenic organism. However, as recent evidence suggests that EcN is capable of inducing inflammatory responses in host intestinal epithelial cells, we aimed to investigate potential pathogenic properties of EcN in an in vivo model using various germ-free (GF) mouse strains. With the exception of C3H/HeJZtm mice, which carry a defective toll-like receptor (TLR)4-allele, no lesions were obvious in mice of different strains orally inoculated with EcN for 1 week, although organ cultures (blood, lung, mesenteric lymph node, pancreas, spleen, liver and kidney) tested positive to various degrees. C3H/HeJZtm mice inoculated with EcN became clinically ill and the majority died or had to be euthanized. Organs of all gnotobiotic C3H/HeJZtm mice were positive for EcN by culture; major histological findings were moderate to severe pyogranulomatous serositis, typhlitis and pancreatitis. Histological findings were corroborated by highly elevated tumour necrosis factor (TNF) serum levels. Lesions were not detected in specified pathogen free maintained C3H/HeJZtm mice, GF C3H/HeJ mice lacking the interleukin-10 gene, or GF C3H/HeJZtm mice that were inoculated with E. coli K12 strain MG1655 as a control. In addition, mild histological lesions were detected in Ztm:NMRI mice 3 months after oral inoculation with EcN. This study shows that EcN is capable of displaying a virulent phenotype in GF C3H/HeJZtm mice. Whether this phenotype is linked to the bacterium's probiotic nature should be the focus of further studies.
CitationSensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background. 2008, 89 (1):45-54notInt J Exp Pathol
AffiliationInstitute for Laboratory Animal Science and Central Animal Facility, Hannover Medical School, Hannover, Germany. email@example.com
The following license files are associated with this item:
- Green fluorescent protein for detection of the probiotic microorganism Escherichia coli strain Nissle 1917 (EcN) in vivo.
- Authors: Schultz M, Watzl S, Oelschlaeger TA, Rath HC, Göttl C, Lehn N, Schölmerich J, Linde HJ
- Issue date: 2005 Jun
- Interference of Bifidobacterium choerinum or Escherichia coli Nissle 1917 with Salmonella Typhimurium in gnotobiotic piglets correlates with cytokine patterns in blood and intestine.
- Authors: Splichalova A, Trebichavsky I, Rada V, Vlkova E, Sonnenborn U, Splichal I
- Issue date: 2011 Feb
- Safety of probiotic Escherichia coli strain Nissle 1917 depends on intestinal microbiota and adaptive immunity of the host.
- Authors: Gronbach K, Eberle U, Müller M, Olschläger TA, Dobrindt U, Leithäuser F, Niess JH, Döring G, Reimann J, Autenrieth IB, Frick JS
- Issue date: 2010 Jul
- Effect of antibiotic, probiotic, and human rotavirus infection on colonisation dynamics of defined commensal microbiota in a gnotobiotic pig model.
- Authors: Huang HC, Vlasova AN, Kumar A, Kandasamy S, Fischer DD, Deblais L, Paim FC, Langel SN, Alhamo MA, Rauf A, Shao L, Saif LJ, Rajashekara G
- Issue date: 2018 Jan 29
- Beneficial effects resulting from oral administration of <i>Escherichia coli</i> Nissle 1917 on a chronic colitis model.
- Authors: Souza ELS, Campos CLV, Reis DC, Cassali GD, Generoso SV, Cardoso VN, Azevedo V, Medeiros JD, Fernandes GR, Nicoli JR, Martins FS
- Issue date: 2020 Dec 2