Sensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background.
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Authors
Bleich, AndreSundberg, John P
Smoczek, Anna
von Wasielewski, Reinhard
de Buhr, Maike F
Janus, Lydia M
Julga, Gwen
Ukena, Sya N
Hedrich, Hans-J
Gunzer, Florian
Issue Date
2008-02
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Show full item recordAbstract
Escherichia coli Nissle 1917 (EcN) is a well-characterized probiotic bacterium. Although genomic comparisons of EcN with the uropathogenic E. coli strain CFT073 revealed high degrees of similarity, EcN is generally considered a non-pathogenic organism. However, as recent evidence suggests that EcN is capable of inducing inflammatory responses in host intestinal epithelial cells, we aimed to investigate potential pathogenic properties of EcN in an in vivo model using various germ-free (GF) mouse strains. With the exception of C3H/HeJZtm mice, which carry a defective toll-like receptor (TLR)4-allele, no lesions were obvious in mice of different strains orally inoculated with EcN for 1 week, although organ cultures (blood, lung, mesenteric lymph node, pancreas, spleen, liver and kidney) tested positive to various degrees. C3H/HeJZtm mice inoculated with EcN became clinically ill and the majority died or had to be euthanized. Organs of all gnotobiotic C3H/HeJZtm mice were positive for EcN by culture; major histological findings were moderate to severe pyogranulomatous serositis, typhlitis and pancreatitis. Histological findings were corroborated by highly elevated tumour necrosis factor (TNF) serum levels. Lesions were not detected in specified pathogen free maintained C3H/HeJZtm mice, GF C3H/HeJ mice lacking the interleukin-10 gene, or GF C3H/HeJZtm mice that were inoculated with E. coli K12 strain MG1655 as a control. In addition, mild histological lesions were detected in Ztm:NMRI mice 3 months after oral inoculation with EcN. This study shows that EcN is capable of displaying a virulent phenotype in GF C3H/HeJZtm mice. Whether this phenotype is linked to the bacterium's probiotic nature should be the focus of further studies.Citation
Sensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background. 2008, 89 (1):45-54notInt J Exp PatholAffiliation
Institute for Laboratory Animal Science and Central Animal Facility, Hannover Medical School, Hannover, Germany. bleich.andre@mh-hannover.dePubMed ID
18005134Type
ArticleLanguage
enISSN
1365-2613ae974a485f413a2113503eed53cd6c53
10.1111/j.1365-2613.2007.00560.x
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