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dc.contributor.authorKeil, E
dc.contributor.authorHöcker, R
dc.contributor.authorSchuster, M
dc.contributor.authorEssmann, F
dc.contributor.authorUeffing, N
dc.contributor.authorHoffman, B
dc.contributor.authorLiebermann, D A
dc.contributor.authorPfeffer, K
dc.contributor.authorSchulze-Osthoff, K
dc.contributor.authorSchmitz, I
dc.date.accessioned2013-05-31T11:53:16Z
dc.date.available2013-05-31T11:53:16Z
dc.date.issued2013-02
dc.identifier.citationPhosphorylation of Atg5 by the Gadd45β-MEKK4-p38 pathway inhibits autophagy. 2013, 20 (2):321-32 Cell Death Differ.en_GB
dc.identifier.issn1476-5403
dc.identifier.pmid23059785
dc.identifier.doi10.1038/cdd.2012.129
dc.identifier.urihttp://hdl.handle.net/10033/293134
dc.description.abstractAutophagy is a lysosomal degradation pathway important for cellular homeostasis, mammalian development, cancer and immunity. Many molecular components of autophagy have been identified, but little is known about regulatory mechanisms controlling their effector functions. Here, we show that, in contrast to other p38 MAP kinase activators, the growth arrest and DNA damage 45 beta (Gadd45β)-MAPK/ERK kinase kinase 4 (MEKK4) pathway specifically directs p38 to autophagosomes. This process results in an accumulation of autophagosomes through p38-mediated inhibition of lysosome fusion. Conversely, autophagic flux is increased in p38-deficient fibroblasts and Gadd45β-deficient cells. We further identified the underlying mechanism and demonstrate that phosphorylation of the autophagy regulator autophagy-related (Atg)5 at threonine 75 through p38 is responsible for inhibition of starvation-induced autophagy. Thus, we show for the first time that Atg5 activity is controlled by phosphorylation and, moreover, that the spatial regulation of p38 by Gadd45β/MEKK4 negatively regulates the autophagic process.
dc.language.isoenen
dc.rightsArchived with thanks to Cell death and differentiationen_GB
dc.titlePhosphorylation of Atg5 by the Gadd45β-MEKK4-p38 pathway inhibits autophagy.en
dc.typeArticleen
dc.contributor.departmentInstitute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine-University, Universitätsstr. 1, Düsseldorf, Germany.en_GB
dc.identifier.journalCell death and differentiationen_GB
refterms.dateFOA2013-08-15T00:00:00Z
html.description.abstractAutophagy is a lysosomal degradation pathway important for cellular homeostasis, mammalian development, cancer and immunity. Many molecular components of autophagy have been identified, but little is known about regulatory mechanisms controlling their effector functions. Here, we show that, in contrast to other p38 MAP kinase activators, the growth arrest and DNA damage 45 beta (Gadd45β)-MAPK/ERK kinase kinase 4 (MEKK4) pathway specifically directs p38 to autophagosomes. This process results in an accumulation of autophagosomes through p38-mediated inhibition of lysosome fusion. Conversely, autophagic flux is increased in p38-deficient fibroblasts and Gadd45β-deficient cells. We further identified the underlying mechanism and demonstrate that phosphorylation of the autophagy regulator autophagy-related (Atg)5 at threonine 75 through p38 is responsible for inhibition of starvation-induced autophagy. Thus, we show for the first time that Atg5 activity is controlled by phosphorylation and, moreover, that the spatial regulation of p38 by Gadd45β/MEKK4 negatively regulates the autophagic process.


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