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dc.contributor.authorEichler, Stefan
dc.contributor.authorChristen, Richard
dc.contributor.authorHöltje, Claudia
dc.contributor.authorWestphal, Petra
dc.contributor.authorBötel, Julia
dc.contributor.authorBrettar, Ingrid
dc.contributor.authorMehling, Arndt
dc.contributor.authorHöfle, Manfred G
dc.date.accessioned2013-06-21T09:25:47Zen
dc.date.available2013-06-21T09:25:47Zen
dc.date.issued2006-03en
dc.identifier.citationComposition and dynamics of bacterial communities of a drinking water supply system as assessed by RNA- and DNA-based 16S rRNA gene fingerprinting. 2006, 72 (3):1858-72 Appl. Environ. Microbiol.en_GB
dc.identifier.issn0099-2240en
dc.identifier.pmid16517632en
dc.identifier.doi10.1128/AEM.72.3.1858-1872.2006en
dc.identifier.urihttp://hdl.handle.net/10033/294325en
dc.description.abstractBacterial community dynamics of a whole drinking water supply system (DWSS) were studied from source to tap. Raw water for this DWSS is provided by two reservoirs with different water characteristics in the Harz mountains of Northern Germany. Samples were taken after different steps of treatment of raw water (i.e., flocculation, sand filtration, and chlorination) and at different points along the supply system to the tap. RNA and DNA were extracted from the sampled water. The 16S rRNA or its genes were partially amplified by reverse transcription-PCR or PCR and analyzed by single-strand conformation polymorphism community fingerprints. The bacterial community structures of the raw water samples from the two reservoirs were very different, but no major changes of these structures occurred after flocculation and sand filtration. Chlorination of the processed raw water strongly affected bacterial community structure, as reflected by the RNA-based fingerprints. This effect was less pronounced for the DNA-based fingerprints. After chlorination, the bacterial community remained rather constant from the storage containers to the tap. Furthermore, the community structure of the tap water did not change substantially for several months. Community composition was assessed by sequencing of abundant bands and phylogenetic analysis of the sequences obtained. The taxonomic compositions of the bacterial communities from both reservoirs were very different at the species level due to their different limnologies. On the other hand, major taxonomic groups, well known to occur in freshwater, such as Alphaproteobacteria, Betaproteobacteria, and Bacteroidetes, were found in both reservoirs. Significant differences in the detection of the major groups were observed between DNA-based and RNA-based fingerprints irrespective of the reservoir. Chlorination of the drinking water seemed to promote growth of nitrifying bacteria. Detailed analysis of the community dynamics of the whole DWSS revealed a significant influence of both source waters on the overall composition of the drinking water microflora and demonstrated the relevance of the raw water microflora for the drinking water microflora provided to the end user.
dc.language.isoenen
dc.rightsArchived with thanks to Applied and environmental microbiologyen_GB
dc.subject.meshBacteriaen_GB
dc.subject.meshDNA Fingerprintingen_GB
dc.subject.meshDNA, Bacterialen_GB
dc.subject.meshDNA, Ribosomalen_GB
dc.subject.meshDrinkingen_GB
dc.subject.meshEcosystemen_GB
dc.subject.meshFresh Wateren_GB
dc.subject.meshGenes, rRNAen_GB
dc.subject.meshMolecular Sequence Dataen_GB
dc.subject.meshPolymorphism, Single-Stranded Conformationalen_GB
dc.subject.meshRNA, Ribosomal, 16Sen_GB
dc.subject.meshSequence Analysis, DNAen_GB
dc.subject.meshWater Supplyen_GB
dc.titleComposition and dynamics of bacterial communities of a drinking water supply system as assessed by RNA- and DNA-based 16S rRNA gene fingerprinting.en
dc.typeArticleen
dc.contributor.departmentDepartment of Environmental Microbiology, GBF-German Research Center for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.en_GB
dc.identifier.journalApplied and environmental microbiologyen_GB
refterms.dateFOA2018-06-13T21:42:24Z
html.description.abstractBacterial community dynamics of a whole drinking water supply system (DWSS) were studied from source to tap. Raw water for this DWSS is provided by two reservoirs with different water characteristics in the Harz mountains of Northern Germany. Samples were taken after different steps of treatment of raw water (i.e., flocculation, sand filtration, and chlorination) and at different points along the supply system to the tap. RNA and DNA were extracted from the sampled water. The 16S rRNA or its genes were partially amplified by reverse transcription-PCR or PCR and analyzed by single-strand conformation polymorphism community fingerprints. The bacterial community structures of the raw water samples from the two reservoirs were very different, but no major changes of these structures occurred after flocculation and sand filtration. Chlorination of the processed raw water strongly affected bacterial community structure, as reflected by the RNA-based fingerprints. This effect was less pronounced for the DNA-based fingerprints. After chlorination, the bacterial community remained rather constant from the storage containers to the tap. Furthermore, the community structure of the tap water did not change substantially for several months. Community composition was assessed by sequencing of abundant bands and phylogenetic analysis of the sequences obtained. The taxonomic compositions of the bacterial communities from both reservoirs were very different at the species level due to their different limnologies. On the other hand, major taxonomic groups, well known to occur in freshwater, such as Alphaproteobacteria, Betaproteobacteria, and Bacteroidetes, were found in both reservoirs. Significant differences in the detection of the major groups were observed between DNA-based and RNA-based fingerprints irrespective of the reservoir. Chlorination of the drinking water seemed to promote growth of nitrifying bacteria. Detailed analysis of the community dynamics of the whole DWSS revealed a significant influence of both source waters on the overall composition of the drinking water microflora and demonstrated the relevance of the raw water microflora for the drinking water microflora provided to the end user.


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