Freeze-drying as a preserving preparation technique for in vitro testing of human skin.
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Issue Date
2013-01
Metadata
Show full item recordAbstract
In vitro testing of drugs with excised human skin is a valuable prerequisite for clinical studies. However, the analysis of excised human skin presents several obstacles. Ongoing drug diffusion, microbial growth and changes in hydration state influence the results of drug penetration studies. In this work, we evaluate freeze-drying as a preserving preparation method for skin samples to overcome these obstacles. We analyse excised human skin before and after freeze-drying and compare these results with human skin in vivo. Based on comprehensive thermal and spectroscopic analysis, we demonstrate comparability to in vivo conditions and exclude significant changes within the skin samples due to freeze-drying. Furthermore, we show that freeze-drying after skin incubation with drugs prevents growth of drug crystals on the skin surface due to drying effects. In conclusion, we introduce freeze-drying as a preserving preparation technique for in vitro testing of human skin.Citation
Freeze-drying as a preserving preparation technique for in vitro testing of human skin. 2013, 22 (1):54-6 Exp. Dermatol.Journal
Experimental dermatologyPubMed ID
23278895Type
ArticleLanguage
enISSN
1600-0625ae974a485f413a2113503eed53cd6c53
10.1111/exd.12058
Scopus Count
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