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dc.contributor.authorFranzen, Lutz
dc.contributor.authorVidlářová, Lucie
dc.contributor.authorKostka, Karl-Heinz
dc.contributor.authorSchaefer, Ulrich F
dc.contributor.authorWindbergs, Maike
dc.date.accessioned2013-06-24T14:25:15Z
dc.date.available2013-06-24T14:25:15Z
dc.date.issued2013-01
dc.identifier.citationFreeze-drying as a preserving preparation technique for in vitro testing of human skin. 2013, 22 (1):54-6 Exp. Dermatol.en_GB
dc.identifier.issn1600-0625
dc.identifier.pmid23278895
dc.identifier.doi10.1111/exd.12058
dc.identifier.urihttp://hdl.handle.net/10033/294443
dc.description.abstractIn vitro testing of drugs with excised human skin is a valuable prerequisite for clinical studies. However, the analysis of excised human skin presents several obstacles. Ongoing drug diffusion, microbial growth and changes in hydration state influence the results of drug penetration studies. In this work, we evaluate freeze-drying as a preserving preparation method for skin samples to overcome these obstacles. We analyse excised human skin before and after freeze-drying and compare these results with human skin in vivo. Based on comprehensive thermal and spectroscopic analysis, we demonstrate comparability to in vivo conditions and exclude significant changes within the skin samples due to freeze-drying. Furthermore, we show that freeze-drying after skin incubation with drugs prevents growth of drug crystals on the skin surface due to drying effects. In conclusion, we introduce freeze-drying as a preserving preparation technique for in vitro testing of human skin.
dc.language.isoenen
dc.rightsArchived with thanks to Experimental dermatologyen_GB
dc.titleFreeze-drying as a preserving preparation technique for in vitro testing of human skin.en
dc.typeArticleen
dc.identifier.journalExperimental dermatologyen_GB
refterms.dateFOA2014-02-15T00:00:00Z
html.description.abstractIn vitro testing of drugs with excised human skin is a valuable prerequisite for clinical studies. However, the analysis of excised human skin presents several obstacles. Ongoing drug diffusion, microbial growth and changes in hydration state influence the results of drug penetration studies. In this work, we evaluate freeze-drying as a preserving preparation method for skin samples to overcome these obstacles. We analyse excised human skin before and after freeze-drying and compare these results with human skin in vivo. Based on comprehensive thermal and spectroscopic analysis, we demonstrate comparability to in vivo conditions and exclude significant changes within the skin samples due to freeze-drying. Furthermore, we show that freeze-drying after skin incubation with drugs prevents growth of drug crystals on the skin surface due to drying effects. In conclusion, we introduce freeze-drying as a preserving preparation technique for in vitro testing of human skin.


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