Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe heterogeneous collection of nucleosome remodelling and deacetylation (NuRD) complexes can be grouped into the MBD2- or MBD3-containing complexes MBD2-NuRD and MBD3-NuRD. MBD2 is known to bind to methylated CpG sequences in vitro in contrast to MBD3. Although functional differences have been described, a direct comparison of MBD2 and MBD3 in respect to genome-wide binding and function has been lacking. Here, we show that MBD2-NuRD, in contrast to MBD3-NuRD, converts open chromatin with euchromatic histone modifications into tightly compacted chromatin with repressive histone marks. Genome-wide, a strong enrichment for MBD2 at methylated CpG sequences is found, whereas CpGs bound by MBD3 are devoid of methylation. MBD2-bound genes are generally lower expressed as compared with MBD3-bound genes. When depleting cells for MBD2, the MBD2-bound genes increase their activity, whereas MBD2 plus MBD3-bound genes reduce their activity. Most strikingly, MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes.
CitationDifferential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences. 2013, 41 (5):3010-21 Nucleic Acids Res.
AffiliationInstitute for Genetics, Justus-Liebig-University, D35392 Giessen, Germany.
JournalNucleic acids research
The following license files are associated with this item:
- MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing.
- Authors: Jin SG, Jiang CL, Rauch T, Li H, Pfeifer GP
- Issue date: 2005 Apr 1
- Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development.
- Authors: Hendrich B, Guy J, Ramsahoye B, Wilson VA, Bird A
- Issue date: 2001 Mar 15
- MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties.
- Authors: Le Guezennec X, Vermeulen M, Brinkman AB, Hoeijmakers WA, Cohen A, Lasonder E, Stunnenberg HG
- Issue date: 2006 Feb
- MBD3L1 is a transcriptional repressor that interacts with methyl-CpG-binding protein 2 (MBD2) and components of the NuRD complex.
- Authors: Jiang CL, Jin SG, Pfeifer GP
- Issue date: 2004 Dec 10
- DNA methylation directs genomic localization of Mbd2 and Mbd3 in embryonic stem cells.
- Authors: Hainer SJ, McCannell KN, Yu J, Ee LS, Zhu LJ, Rando OJ, Fazzio TG
- Issue date: 2016 Nov 16