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dc.contributor.authorGünther, Katharina
dc.contributor.authorRust, Mareike
dc.contributor.authorLeers, Joerg
dc.contributor.authorBoettger, Thomas
dc.contributor.authorScharfe, Maren
dc.contributor.authorJarek, Michael
dc.contributor.authorBartkuhn, Marek
dc.contributor.authorRenkawitz, Rainer
dc.date.accessioned2013-08-22T14:30:57Z
dc.date.available2013-08-22T14:30:57Z
dc.date.issued2013-03-01
dc.identifier.citationDifferential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences. 2013, 41 (5):3010-21 Nucleic Acids Res.en_GB
dc.identifier.issn1362-4962
dc.identifier.pmid23361464
dc.identifier.doi10.1093/nar/gkt035
dc.identifier.urihttp://hdl.handle.net/10033/299507
dc.description.abstractThe heterogeneous collection of nucleosome remodelling and deacetylation (NuRD) complexes can be grouped into the MBD2- or MBD3-containing complexes MBD2-NuRD and MBD3-NuRD. MBD2 is known to bind to methylated CpG sequences in vitro in contrast to MBD3. Although functional differences have been described, a direct comparison of MBD2 and MBD3 in respect to genome-wide binding and function has been lacking. Here, we show that MBD2-NuRD, in contrast to MBD3-NuRD, converts open chromatin with euchromatic histone modifications into tightly compacted chromatin with repressive histone marks. Genome-wide, a strong enrichment for MBD2 at methylated CpG sequences is found, whereas CpGs bound by MBD3 are devoid of methylation. MBD2-bound genes are generally lower expressed as compared with MBD3-bound genes. When depleting cells for MBD2, the MBD2-bound genes increase their activity, whereas MBD2 plus MBD3-bound genes reduce their activity. Most strikingly, MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes.
dc.language.isoenen
dc.rightsArchived with thanks to Nucleic acids researchen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshBinding Sitesen_GB
dc.subject.meshCell Lineen_GB
dc.subject.meshCpG Islandsen_GB
dc.subject.meshDNA Methylationen_GB
dc.subject.meshDNA-Binding Proteinsen_GB
dc.subject.meshEpigenesis, Geneticen_GB
dc.subject.meshEuchromatinen_GB
dc.subject.meshExonsen_GB
dc.subject.meshGenome, Humanen_GB
dc.subject.meshHumansen_GB
dc.subject.meshPromoter Regions, Geneticen_GB
dc.subject.meshProtein Bindingen_GB
dc.subject.meshProtein Isoformsen_GB
dc.subject.meshProtein Transporten_GB
dc.subject.meshRatsen_GB
dc.subject.meshTranscription Initiation Siteen_GB
dc.titleDifferential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences.en
dc.typeArticleen
dc.contributor.departmentInstitute for Genetics, Justus-Liebig-University, D35392 Giessen, Germany.en_GB
dc.identifier.journalNucleic acids researchen_GB
refterms.dateFOA2018-06-12T21:20:40Z
html.description.abstractThe heterogeneous collection of nucleosome remodelling and deacetylation (NuRD) complexes can be grouped into the MBD2- or MBD3-containing complexes MBD2-NuRD and MBD3-NuRD. MBD2 is known to bind to methylated CpG sequences in vitro in contrast to MBD3. Although functional differences have been described, a direct comparison of MBD2 and MBD3 in respect to genome-wide binding and function has been lacking. Here, we show that MBD2-NuRD, in contrast to MBD3-NuRD, converts open chromatin with euchromatic histone modifications into tightly compacted chromatin with repressive histone marks. Genome-wide, a strong enrichment for MBD2 at methylated CpG sequences is found, whereas CpGs bound by MBD3 are devoid of methylation. MBD2-bound genes are generally lower expressed as compared with MBD3-bound genes. When depleting cells for MBD2, the MBD2-bound genes increase their activity, whereas MBD2 plus MBD3-bound genes reduce their activity. Most strikingly, MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes.


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