Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences.
dc.contributor.author | Günther, Katharina | |
dc.contributor.author | Rust, Mareike | |
dc.contributor.author | Leers, Joerg | |
dc.contributor.author | Boettger, Thomas | |
dc.contributor.author | Scharfe, Maren | |
dc.contributor.author | Jarek, Michael | |
dc.contributor.author | Bartkuhn, Marek | |
dc.contributor.author | Renkawitz, Rainer | |
dc.date.accessioned | 2013-08-22T14:30:57Z | |
dc.date.available | 2013-08-22T14:30:57Z | |
dc.date.issued | 2013-03-01 | |
dc.identifier.citation | Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences. 2013, 41 (5):3010-21 Nucleic Acids Res. | en_GB |
dc.identifier.issn | 1362-4962 | |
dc.identifier.pmid | 23361464 | |
dc.identifier.doi | 10.1093/nar/gkt035 | |
dc.identifier.uri | http://hdl.handle.net/10033/299507 | |
dc.description.abstract | The heterogeneous collection of nucleosome remodelling and deacetylation (NuRD) complexes can be grouped into the MBD2- or MBD3-containing complexes MBD2-NuRD and MBD3-NuRD. MBD2 is known to bind to methylated CpG sequences in vitro in contrast to MBD3. Although functional differences have been described, a direct comparison of MBD2 and MBD3 in respect to genome-wide binding and function has been lacking. Here, we show that MBD2-NuRD, in contrast to MBD3-NuRD, converts open chromatin with euchromatic histone modifications into tightly compacted chromatin with repressive histone marks. Genome-wide, a strong enrichment for MBD2 at methylated CpG sequences is found, whereas CpGs bound by MBD3 are devoid of methylation. MBD2-bound genes are generally lower expressed as compared with MBD3-bound genes. When depleting cells for MBD2, the MBD2-bound genes increase their activity, whereas MBD2 plus MBD3-bound genes reduce their activity. Most strikingly, MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to Nucleic acids research | en_GB |
dc.subject.mesh | Animals | en_GB |
dc.subject.mesh | Binding Sites | en_GB |
dc.subject.mesh | Cell Line | en_GB |
dc.subject.mesh | CpG Islands | en_GB |
dc.subject.mesh | DNA Methylation | en_GB |
dc.subject.mesh | DNA-Binding Proteins | en_GB |
dc.subject.mesh | Epigenesis, Genetic | en_GB |
dc.subject.mesh | Euchromatin | en_GB |
dc.subject.mesh | Exons | en_GB |
dc.subject.mesh | Genome, Human | en_GB |
dc.subject.mesh | Humans | en_GB |
dc.subject.mesh | Promoter Regions, Genetic | en_GB |
dc.subject.mesh | Protein Binding | en_GB |
dc.subject.mesh | Protein Isoforms | en_GB |
dc.subject.mesh | Protein Transport | en_GB |
dc.subject.mesh | Rats | en_GB |
dc.subject.mesh | Transcription Initiation Site | en_GB |
dc.title | Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences. | en |
dc.type | Article | en |
dc.contributor.department | Institute for Genetics, Justus-Liebig-University, D35392 Giessen, Germany. | en_GB |
dc.identifier.journal | Nucleic acids research | en_GB |
refterms.dateFOA | 2018-06-12T21:20:40Z | |
html.description.abstract | The heterogeneous collection of nucleosome remodelling and deacetylation (NuRD) complexes can be grouped into the MBD2- or MBD3-containing complexes MBD2-NuRD and MBD3-NuRD. MBD2 is known to bind to methylated CpG sequences in vitro in contrast to MBD3. Although functional differences have been described, a direct comparison of MBD2 and MBD3 in respect to genome-wide binding and function has been lacking. Here, we show that MBD2-NuRD, in contrast to MBD3-NuRD, converts open chromatin with euchromatic histone modifications into tightly compacted chromatin with repressive histone marks. Genome-wide, a strong enrichment for MBD2 at methylated CpG sequences is found, whereas CpGs bound by MBD3 are devoid of methylation. MBD2-bound genes are generally lower expressed as compared with MBD3-bound genes. When depleting cells for MBD2, the MBD2-bound genes increase their activity, whereas MBD2 plus MBD3-bound genes reduce their activity. Most strikingly, MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes. |