Human β-Defensin 2 Induces Extracellular Accumulation of Adenosine in Escherichia coli.
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AuthorsEstrela, Andreia Bergamo
Gutierrez, Maximiliano Gabriel
MetadataShow full item record
AbstractHuman β-defensins are host defense peptides performing antimicrobial as well as immunomodulatory functions. The present study investigated whether treatment of Escherichia coli with human β-defensin 2 could generate extracellular molecules of relevance for immune regulation. Mass spectrometry analysis of bacterial supernatants detected the accumulation of purine nucleosides triggered by β-defensin 2 treatment. Other cationic antimicrobial peptides tested presented variable outcomes with regard to extracellular adenosine accumulation; human β-defensin 2 was the most efficient at inducing this response. Structural and biochemical evidence indicated that a mechanism other than plain lysis was involved in the observed phenomenon. By use of isotope ((13)C) labeling, extracellular adenosine was found to be derived from preexistent RNA, and a direct interaction between the peptide and bacterial nucleic acid was documented for the first time for β-defensin 2. Taken together, the data suggest that defensin activity on a bacterial target may alter local levels of adenosine, a well-known immunomodulator influencing inflammatory processes.
CitationHuman β-Defensin 2 Induces Extracellular Accumulation of Adenosine in Escherichia coli. 2013, 57 (9):4387-93 Antimicrob. Agents Chemother.
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