Generation of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Hornef, Mathias W
MetadataShow full item record
AbstractCell lines derived from the small intestine that reflect authentic properties of the originating intestinal epithelium are of high value for studies on mucosal immunology and host microbial homeostasis. A novel immortalization procedure was applied to generate continuously proliferating cell lines from murine E19 embryonic small intestinal tissue. The obtained cell lines form a tight and polarized epithelial cell layer, display characteristic tight junction, microvilli and surface protein expression and generate increasing transepithelial electrical resistance during in vitro culture. Significant up-regulation of Cxcl2 and Cxcl5 chemokine expression upon exposure to defined microbial innate immune stimuli and endogenous cytokines is observed. Cell lines were also generated from a transgenic interferon reporter (Mx2-Luciferase) mouse, allowing reporter technology-based quantification of the cellular response to type I and III interferon. Thus, the newly created cell lines mimic properties of the natural epithelium and can be used for diverse studies including testing of the absorption of drug candidates. The reproducibility of the method to create such cell lines from wild type and transgenic mice provides a new tool to study molecular and cellular processes of the epithelial barrier.
CitationGeneration of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions. 2013, 8 (8):e72700 PLoS ONE
AffiliationDepartment of Gene Regulation and Differentiation, Helmholtz Centre for Infection Research, Braunschweig, Germany.
The following license files are associated with this item:
- Intestinal epithelial cells: regulators of barrier function and immune homeostasis.
- Authors: Peterson LW, Artis D
- Issue date: 2014 Mar
- Innate immune signalling at the intestinal epithelium in homeostasis and disease.
- Authors: Pott J, Hornef M
- Issue date: 2012 Aug
- Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets.
- Authors: Chowdhury SR, King DE, Willing BP, Band MR, Beever JE, Lane AB, Loor JJ, Marini JC, Rund LA, Schook LB, Van Kessel AG, Gaskins HR
- Issue date: 2007 Jul 5
- Gene expression study of two widely used pig intestinal epithelial cell lines: IPEC-J2 and IPI-2I.
- Authors: Mariani V, Palermo S, Fiorentini S, Lanubile A, Giuffra E
- Issue date: 2009 Oct 15
- A method for high purity intestinal epithelial cell culture from adult human and murine tissues for the investigation of innate immune function.
- Authors: Graves CL, Harden SW, LaPato M, Nelson M, Amador B, Sorenson H, Frazier CJ, Wallet SM
- Issue date: 2014 Dec 1