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Authors
Roy, BishnudeoAgarwal, Shiwani
Brennecke, Anne-Margarete
Krey, Martina
Pabst, Oliver
Düber, Sandra
Weiss, Siegfried
Issue Date
2013-08
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Show full item recordAbstract
In mice, B-1 (B1a/B1b) cells are mainly located in the peritoneal cavity. B-1 cells are well known for their role in the early stages of Ab-mediated immune responses against pathogenic invasion as well as for the production of natural IgM antibodies. Although such B cells have been claimed to give rise to intestinal plasma cells producing IgA, a clear role of B-1 cells in IgA production in the gut-associated tissues is still not defined. Here, we employed the transgenic L2 mouse model characterized by the lack of B-2 cells and presence of B-1 cells as major B-cell subpopulation. The oligoclonality of the Ab repertoire in this mouse allowed us to take typical B1a cell VH sequences as indicators of the presence of IgM-producing B-1a cells in Peyer's patches as well as in lamina propria. However, amongst the IgAVH sequences recovered from the same tissues, none of the sequences showed B1a-cell specificity. Interestingly, all IgAVH sequences derived from the lamina propria of L2 mice displayed extensive numbers of nucleotide exchanges, indicating somatic hypermutation, and affinity maturation. This suggests that the contribution of natural unmutated IgA by B-1a cells to intestinal immunity is negligible.Citation
B-1-cell subpopulations contribute differently to gut immunity. 2013, 43 (8):2023-32 Eur. J. Immunol.Affiliation
Molecular Immunology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.Journal
European journal of immunologyPubMed ID
23677546Type
ArticleLanguage
enISSN
1521-4141ae974a485f413a2113503eed53cd6c53
10.1002/eji.201243070
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