Complete sequence of the suicide vector pJP5603.
dc.contributor.author | Riedel, Thomas | |
dc.contributor.author | Rohlfs, Meike | |
dc.contributor.author | Buchholz, Ina | |
dc.contributor.author | Wagner-Döbler, Irene | |
dc.contributor.author | Reck, Michael | |
dc.date.accessioned | 2013-10-31T12:38:37Z | |
dc.date.available | 2013-10-31T12:38:37Z | |
dc.date.issued | 2013-01 | |
dc.identifier.citation | Complete sequence of the suicide vector pJP5603. 2013, 69 (1):104-7 Plasmid | en |
dc.identifier.issn | 1095-9890 | |
dc.identifier.pmid | 22902299 | |
dc.identifier.doi | 10.1016/j.plasmid.2012.07.005 | |
dc.identifier.uri | http://hdl.handle.net/10033/304813 | |
dc.description.abstract | We have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to Plasmid | en |
dc.subject.mesh | Bacterial Proteins | en |
dc.subject.mesh | Base Sequence | en |
dc.subject.mesh | Chromosomes | en |
dc.subject.mesh | Cloning, Molecular | en |
dc.subject.mesh | DNA Replication | en |
dc.subject.mesh | Escherichia coli | en |
dc.subject.mesh | Genetic Vectors | en |
dc.subject.mesh | Homologous Recombination | en |
dc.subject.mesh | Kanamycin | en |
dc.subject.mesh | Lac Operon | en |
dc.subject.mesh | Mutagenesis, Site-Directed | en |
dc.subject.mesh | Plasmids | en |
dc.title | Complete sequence of the suicide vector pJP5603. | en |
dc.type | Article | en |
dc.contributor.department | Helmholtz-Centre for Infection Research, Group Microbial Communication, Braunschweig, Germany. tri07@helmholtz-hzi.de | en |
dc.identifier.journal | Plasmid | en |
refterms.dateFOA | 2018-06-13T05:37:36Z | |
html.description.abstract | We have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments. |