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dc.contributor.authorRiedel, Thomas
dc.contributor.authorRohlfs, Meike
dc.contributor.authorBuchholz, Ina
dc.contributor.authorWagner-Döbler, Irene
dc.contributor.authorReck, Michael
dc.date.accessioned2013-10-31T12:38:37Z
dc.date.available2013-10-31T12:38:37Z
dc.date.issued2013-01
dc.identifier.citationComplete sequence of the suicide vector pJP5603. 2013, 69 (1):104-7 Plasmiden
dc.identifier.issn1095-9890
dc.identifier.pmid22902299
dc.identifier.doi10.1016/j.plasmid.2012.07.005
dc.identifier.urihttp://hdl.handle.net/10033/304813
dc.description.abstractWe have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments.
dc.language.isoenen
dc.rightsArchived with thanks to Plasmiden
dc.subject.meshBacterial Proteinsen
dc.subject.meshBase Sequenceen
dc.subject.meshChromosomesen
dc.subject.meshCloning, Molecularen
dc.subject.meshDNA Replicationen
dc.subject.meshEscherichia colien
dc.subject.meshGenetic Vectorsen
dc.subject.meshHomologous Recombinationen
dc.subject.meshKanamycinen
dc.subject.meshLac Operonen
dc.subject.meshMutagenesis, Site-Directeden
dc.subject.meshPlasmidsen
dc.titleComplete sequence of the suicide vector pJP5603.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Centre for Infection Research, Group Microbial Communication, Braunschweig, Germany. tri07@helmholtz-hzi.deen
dc.identifier.journalPlasmiden
refterms.dateFOA2018-06-13T05:37:36Z
html.description.abstractWe have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments.


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