Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis.

Strobel, Tina; Schmidt, Yvonne; Linnenbrink, Anton; Luzhetskyy, Andriy N; Luzhetska, Marta; Taguchi, Takaaki; Brötz, Elke; Paululat, Thomas; Stasevych, Maryna; Stanko, Oleg; Novikov, Volodymyr; Bechthold, Andreas

dc.contributor.author	Strobel, Tina
dc.contributor.author	Schmidt, Yvonne
dc.contributor.author	Linnenbrink, Anton
dc.contributor.author	Luzhetskyy, Andriy N
dc.contributor.author	Luzhetska, Marta
dc.contributor.author	Taguchi, Takaaki
dc.contributor.author	Brötz, Elke
dc.contributor.author	Paululat, Thomas
dc.contributor.author	Stasevych, Maryna
dc.contributor.author	Stanko, Oleg
dc.contributor.author	Novikov, Volodymyr
dc.contributor.author	Bechthold, Andreas
dc.date.accessioned	2013-10-30T15:19:00Z	en
dc.date.available	2013-10-30T15:19:00Z	en
dc.date.issued	2013-09	en
dc.identifier.citation	Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis. 2013, 79 (17):5224-32 Appl. Environ. Microbiol.	en
dc.identifier.issn	1098-5336	en
dc.identifier.pmid	23793643	en
dc.identifier.doi	10.1128/AEM.01652-13	en
dc.identifier.uri	http://hdl.handle.net/10033/304824	en
dc.description.abstract	Saccharothrix espanaensis is a member of the order Actinomycetales. The genome of the strain has been sequenced recently, revealing 106 glycosyltransferase genes. In this paper, we report the detection of a glycosyltransferase from Saccharothrix espanaensis which is able to rhamnosylate different phenolic compounds targeting different positions of the molecules. The gene encoding the flexible glycosyltransferase is not located close to a natural product biosynthetic gene cluster. Therefore, the native function of this enzyme might be not the biosynthesis of a secondary metabolite but the glycosylation of internal and external natural products as part of a defense mechanism.
dc.language.iso	en	en
dc.rights	Archived with thanks to Applied and environmental microbiology	en
dc.title	Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis.	en
dc.type	Article	en
dc.contributor.department	Albert Ludwigs University of Freiburg, Department of Pharmaceutical Biology and Biotechnology, Freiburg, Germany.	en
dc.identifier.journal	Applied and environmental microbiology	en
refterms.dateFOA	2018-06-13T09:11:45Z
html.description.abstract	Saccharothrix espanaensis is a member of the order Actinomycetales. The genome of the strain has been sequenced recently, revealing 106 glycosyltransferase genes. In this paper, we report the detection of a glycosyltransferase from Saccharothrix espanaensis which is able to rhamnosylate different phenolic compounds targeting different positions of the molecules. The gene encoding the flexible glycosyltransferase is not located close to a natural product biosynthetic gene cluster. Therefore, the native function of this enzyme might be not the biosynthesis of a secondary metabolite but the glycosylation of internal and external natural products as part of a defense mechanism.