Human Host Defense Peptide LL-37 Stimulates Virulence Factor Production and Adaptive Resistance in Pseudomonas aeruginosa.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractA multitude of different virulence factors as well as the ability to rapidly adapt to adverse environmental conditions are important features for the high pathogenicity of Pseudomonas aeruginosa. Both virulence and adaptive resistance are tightly controlled by a complex regulatory network and respond to external stimuli, such as host signals or antibiotic stress, in a highly specific manner. Here, we demonstrate that physiological concentrations of the human host defense peptide LL-37 promote virulence factor production as well as an adaptive resistance against fluoroquinolone and aminoglycoside antibiotics in P. aeruginosa PAO1. Microarray analyses of P. aeruginosa cells exposed to LL-37 revealed an upregulation of gene clusters involved in the production of quorum sensing molecules and secreted virulence factors (PQS, phenazine, hydrogen cyanide (HCN), elastase and rhamnolipids) and in lipopolysaccharide (LPS) modification as well as an induction of genes encoding multidrug efflux pumps MexCD-OprJ and MexGHI-OpmD. Accordingly, we detected significantly elevated levels of toxic metabolites and proteases in bacterial supernatants after LL-37 treatment. Pre-incubation of bacteria with LL-37 for 2 h led to a decreased susceptibility towards gentamicin and ciprofloxacin. Quantitative Realtime PCR results using a PAO1-pqsE mutant strain present evidence that the quinolone response protein and virulence regulator PqsE may be implicated in the regulation of the observed phenotype in response to LL-37. Further experiments with synthetic cationic antimicrobial peptides IDR-1018, 1037 and HHC-36 showed no induction of pqsE expression, suggesting a new role of PqsE as highly specific host stress sensor.
CitationHuman Host Defense Peptide LL-37 Stimulates Virulence Factor Production and Adaptive Resistance in Pseudomonas aeruginosa. 2013, 8 (12):e82240 PLoS ONE
AffiliationResearch group genomeanalytics, Helmholtz Centre for infection research, Braunschweig, Germany
The following license files are associated with this item:
- Uncoupled Quorum Sensing Modulates the Interplay of Virulence and Resistance in a Multidrug-Resistant Clinical <i>Pseudomonas aeruginosa</i> Isolate Belonging to the MLST550 Clonal Complex.
- Authors: Cao H, Xia T, Li Y, Xu Z, Bougouffa S, Lo YK, Bajic VB, Luo H, Woo PCY, Yan A
- Issue date: 2019 Apr
- Identification of FDA-approved antivirulence drugs targeting the <i>Pseudomonas aeruginosa</i> quorum sensing effector protein PqsE.
- Authors: Baldelli V, D'Angelo F, Pavoncello V, Fiscarelli EV, Visca P, Rampioni G, Leoni L
- Issue date: 2020 Dec
- C-type natriuretic peptide modulates quorum sensing molecule and toxin production in Pseudomonas aeruginosa.
- Authors: Blier AS, Veron W, Bazire A, Gerault E, Taupin L, Vieillard J, Rehel K, Dufour A, Le Derf F, Orange N, Hulen C, Feuilloley MGJ, Lesouhaitier O
- Issue date: 2011 Jul
- The PqsE-RhlR Interaction Regulates RhlR DNA Binding to Control Virulence Factor Production in <i>Pseudomonas aeruginosa</i>.
- Authors: Simanek KA, Taylor IR, Richael EK, Lasek-Nesselquist E, Bassler BL, Paczkowski JE
- Issue date: 2022 Feb 23
- Resistance and virulence of Pseudomonas aeruginosa clinical strains overproducing the MexCD-OprJ efflux pump.
- Authors: Jeannot K, Elsen S, Köhler T, Attree I, van Delden C, Plésiat P
- Issue date: 2008 Jul