Human and animal isolates of Yersinia enterocolitica show significant serotype-specific colonization and host-specific immune defense properties.
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Schaake, JuliaKronshage, Malte
Uliczka, Frank
Rohde, Manfred
Knuuti, Tobias
Strauch, Eckhard
Fruth, Angelika
Wos-Oxley, Melissa
Dersch, Petra
Issue Date
2013-11
Metadata
Show full item recordAbstract
Yersinia enterocolitica is a human pathogen that is ubiquitous in livestock, especially pigs. The bacteria are able to colonize the intestinal tract of a variety of mammalian hosts, but the severity of induced gut-associated diseases (yersiniosis) differs significantly between hosts. To gain more information about the individual virulence determinants that contribute to colonization and induction of immune responses in different hosts, we analyzed and compared the interactions of different human- and animal-derived isolates of serotypes O:3, O:5,27, O:8, and O:9 with murine, porcine, and human intestinal cells and macrophages. The examined strains exhibited significant serotype-specific cell binding and entry characteristics, but adhesion and uptake into different host cells were not host specific and were independent of the source of the isolate. In contrast, survival and replication within macrophages and the induced proinflammatory response differed between murine, porcine, and human macrophages, suggesting a host-specific immune response. In fact, similar levels of the proinflammatory cytokine macrophage inflammatory protein 2 (MIP-2) were secreted by murine bone marrow-derived macrophages with all tested isolates, but the equivalent interleukin-8 (IL-8) response of porcine bone marrow-derived macrophages was strongly serotype specific and considerably lower in O:3 than in O:8 strains. In addition, all tested Y. enterocolitica strains caused a considerably higher level of secretion of the anti-inflammatory cytokine IL-10 by porcine than by murine macrophages. This could contribute to limiting the severity of the infection (in particular of serotype O:3 strains) in pigs, which are the primary reservoir of Y. enterocolitica strains pathogenic to humans.Citation
Human and animal isolates of Yersinia enterocolitica show significant serotype-specific colonization and host-specific immune defense properties. 2013, 81 (11):4013-25 Infect. Immun.Affiliation
Dept. of molecular infection biology, Helmholtz Centre for infection biology, Inhoffenstr. 7, D-38124 Braunschweig, Germany.Journal
Infection and immunityPubMed ID
23959720Type
ArticleLanguage
enISSN
1098-5522ae974a485f413a2113503eed53cd6c53
10.1128/IAI.00572-13
Scopus Count
The following license files are associated with this item:
Related articles
- Unique virulence properties of Yersinia enterocolitica O:3--an emerging zoonotic pathogen using pigs as preferred reservoir host.
- Authors: Valentin-Weigand P, Heesemann J, Dersch P
- Issue date: 2014 Oct
- Unique cell adhesion and invasion properties of Yersinia enterocolitica O:3, the most frequent cause of human Yersiniosis.
- Authors: Uliczka F, Pisano F, Schaake J, Stolz T, Rohde M, Fruth A, Strauch E, Skurnik M, Batzilla J, Rakin A, Heesemann J, Dersch P
- Issue date: 2011 Jul
- Yersinia enterocolitica isolates of differing biotypes from humans and animals are adherent, invasive and persist in macrophages, but differ in cytokine secretion profiles in vitro.
- Authors: McNally A, Dalton T, Ragione RM, Stapleton K, Manning G, Newell DG
- Issue date: 2006 Dec
- Contribution of the major secreted yops of Yersinia enterocolitica O:8 to pathogenicity in the mouse infection model.
- Authors: Trülzsch K, Sporleder T, Igwe EI, Rüssmann H, Heesemann J
- Issue date: 2004 Sep
- Comparison of the cytokine immune response to pathogenic Yersinia enterocolitica bioserotype 1B/O:8 and 2/O:9 in susceptible BALB/C and resistant C57BL/6 mice.
- Authors: Wang X, Gu W, Qiu H, Xia S, Zheng H, Xiao Y, Liang J, Jing H
- Issue date: 2013 Oct