A replication study for genome-wide gene expression levels in two layer lines elucidates differentially expressed genes of pathways involved in bone remodeling and immune responsiveness.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe current replication study confirmed significant differences in gene expression profiles of the cerebrum among the two commercial layer lines Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB). Microarray analyses were performed for 30 LSL and another 30 LB laying hens kept in the small group housing system Eurovent German. A total of 14,103 microarray probe sets using customized Affymetrix ChiGene-1_0-st Arrays with 20,399 probe sets were differentially expressed among the two layer lines LSL and LB (FDR adjusted P-value <0.05). An at least 2-fold change in expression levels could be observed for 388 of these probe sets. In LSL, 214 of the 388 probe sets were down- and 174 were up-regulated and vice versa for the LB layer line. Among the 174 up-regulated probe sets in LSL, we identified 51 significantly enriched Gene ontology (GO) terms of the biological process category. A total of 63 enriched GO-terms could be identified for the 214 down-regulated probe sets of the layer line LSL. We identified nine genes significantly differentially expressed between the two layer lines in both microarray experiments. These genes play a crucial role in protection of neuronal cells from oxidative stress, bone mineral density and immune response among the two layer lines LSL and LB. Thus, the different regulation of these genes may significantly contribute to phenotypic trait differences among these layer lines. In conclusion, these novel findings provide a basis for further research to improve animal welfare in laying hens and these layer lines may be of general interest as an animal model.
CitationA replication study for genome-wide gene expression levels in two layer lines elucidates differentially expressed genes of pathways involved in bone remodeling and immune responsiveness. 2014, 9 (6):e98350 PLoS ONE
The following license files are associated with this item:
- Differential gene expression from genome-wide microarray analyses distinguishes Lohmann Selected Leghorn and Lohmann Brown layers.
- Authors: Habig C, Geffers R, Distl O
- Issue date: 2012
- Effect of furnished small group housing systems and furnished cages on mortality and causes of death in two layer strains.
- Authors: Weitzenbürger D, Vits A, Hamann H, Distl O
- Issue date: 2005 Oct
- Evaluation of bone strength, keel bone status, plumage condition and egg quality of two layer lines kept in small group housing systems.
- Authors: Habig C, Distl O
- Issue date: 2013
- Daily energy expenditure and water turnover in two breeds of laying hens kept in floor housing.
- Authors: Riek A, Petow S, Speakman JR, Schrader L
- Issue date: 2021 Jan
- Genetic resistance to natural helminth infections in two chicken layer lines.
- Authors: Kaufmann F, Daş G, Preisinger R, Schmutz M, König S, Gauly M
- Issue date: 2011 Mar 10