Expression, purification, crystallization and preliminary X-ray diffraction analysis of a mammalian type 10 adenylyl cyclase.
Kleinboelting et al._final.pdf
allowed publisher's PDF
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
van den Heuvel, Joop
MetadataShow full item record
AbstractThe second messenger cAMP is synthesized in mammals by ten differently regulated adenylyl cyclases (AC1-10). These ACs are grouped into nucleotidyl cyclase class III based on homologies in their catalytic domains. The catalytic domain of AC10 is unique, however, in being activated through direct interaction with calcium and bicarbonate. Here, the production, crystallization and X-ray diffraction analysis of the catalytic domain of human AC10 are described as a basis for structural studies of regulator binding sites and mechanisms. The recombinant protein had high specific AC activity, and crystals of AC10 in space group P63 diffracted to ∼2.0 Å resolution on a synchrotron beamline. A complete diffraction data set revealed unit-cell parameters a = b = 99.65, c = 98.04 Å, indicating one AC10 catalytic domain per asymmetric unit, and confirmed that the obtained crystals are suitable for structure solution and mechanistic studies.
CitationExpression, purification, crystallization and preliminary X-ray diffraction analysis of a mammalian type 10 adenylyl cyclase. 2014, 70 (Pt 4):467-9 Acta Crystallogr F Struct Biol Commun
The following license files are associated with this item:
- Synthesis and degradation of cAMP in Giardia lamblia: possible role and characterization of a nucleotidyl cyclase with a single cyclase homology domain.
- Authors: Saraullo V, Di Siervi N, Jerez B, Davio C, Zurita A
- Issue date: 2017 Nov 21
- Structural analysis of human soluble adenylyl cyclase and crystal structures of its nucleotide complexes-implications for cyclase catalysis and evolution.
- Authors: Kleinboelting S, van den Heuvel J, Steegborn C
- Issue date: 2014 Sep
- Crystallization of complex between soluble domains of adenylyl cyclase and activated Gs alpha.
- Authors: Tesmer JJ, Sunahara RK, Fancy DA, Gilman AG, Sprang SR
- Issue date: 2002
- Structure, mechanism, and regulation of soluble adenylyl cyclases - similarities and differences to transmembrane adenylyl cyclases.
- Authors: Steegborn C
- Issue date: 2014 Dec
- Substrate specificity determinants of class III nucleotidyl cyclases.
- Authors: Bharambe NG, Barathy DV, Syed W, Visweswariah SS, Colaςo M, Misquith S, Suguna K
- Issue date: 2016 Oct