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dc.contributor.authorMontero-Calasanz, Maria Del Carmen
dc.contributor.authorHofner, Benjamin
dc.contributor.authorGöker, Markus
dc.contributor.authorRohde, Manfred
dc.contributor.authorSpröer, Cathrin
dc.contributor.authorHezbri, Karima
dc.contributor.authorGtari, Maher
dc.contributor.authorSchumann, Peter
dc.contributor.authorKlenk, Hans-Peter
dc.date.accessioned2014-09-12T14:17:46Z
dc.date.available2014-09-12T14:17:46Z
dc.date.issued2014
dc.identifier.citationGeodermatophilus poikilotrophi sp. nov.: A Multitolerant Actinomycete Isolated from Dolomitic Marble. 2014, 2014:914767 Biomed Res Inten
dc.identifier.issn2314-6141
dc.identifier.pmid25114928
dc.identifier.doi10.1155/2014/914767
dc.identifier.urihttp://hdl.handle.net/10033/326076
dc.description.abstractA novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- and UV-radiation, designated G18(T), was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15-35°C, at pH 5.5-9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for other representatives of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and small amount of diphosphatidylglycerol. MK-9(H4) was the dominant menaquinone and galactose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C16:0 and iso-C15:0 and the unsaturated C17:1 ω8c and C16:1 ω7c. The 16S rRNA gene showed 97.4-99.1% sequence identity with the other representatives of genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G18(T) is proposed to represent a novel species, Geodermatophilus poikilotrophi. Type strain is G18(T) (= DSM 44209(T) = CCUG 63018(T)). The INSDC accession number is HF970583. The novel R software package lethal was used to compute the lethal doses with confidence intervals resulting from tolerance experiments.
dc.language.isoenen
dc.rightsArchived with thanks to BioMed research internationalen
dc.titleGeodermatophilus poikilotrophi sp. nov.: A Multitolerant Actinomycete Isolated from Dolomitic Marble.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalBioMed research internationalen
refterms.dateFOA2018-06-12T16:51:47Z
html.description.abstractA novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- and UV-radiation, designated G18(T), was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15-35°C, at pH 5.5-9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for other representatives of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and small amount of diphosphatidylglycerol. MK-9(H4) was the dominant menaquinone and galactose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C16:0 and iso-C15:0 and the unsaturated C17:1 ω8c and C16:1 ω7c. The 16S rRNA gene showed 97.4-99.1% sequence identity with the other representatives of genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G18(T) is proposed to represent a novel species, Geodermatophilus poikilotrophi. Type strain is G18(T) (= DSM 44209(T) = CCUG 63018(T)). The INSDC accession number is HF970583. The novel R software package lethal was used to compute the lethal doses with confidence intervals resulting from tolerance experiments.


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