Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsVázquez, Cristina L
Lerner, Thomas R
Bianco, Maria V
Blanco, Federico C
Gutierrez, Maximiliano G
MetadataShow full item record
AbstractSome intracellular bacteria are known to cause long-term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long-term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re-infection and also with the specific expression of stress- and survival-related genes. Our findings identify bacterial traits implicated in the establishment of long-term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments.
CitationExperimental selection of long-term intracellular mycobacteria. 2014, 16 (9):1425-40 Cell. Microbiol.
The following license files are associated with this item:
- Identification and macrophage-activating activity of glycolipids released from intracellular Mycobacterium bovis BCG.
- Authors: Rhoades E, Hsu F, Torrelles JB, Turk J, Chatterjee D, Russell DG
- Issue date: 2003 May
- The Lack of the TetR-Like Repressor Gene BCG_2177c (Rv2160A) May Help Mycobacteria Overcome Intracellular Redox Stress and Survive Longer Inside Macrophages When Surrounded by a Lipid Environment.
- Authors: García-Morales L, Del Portillo P, Anzola JM, Ares MA, Helguera-Repetto AC, Cerna-Cortes JF, Méndez-Tenorio A, García MJ, Otal I, Martín C, Gonzalez-Y-Merchand JA, Rivera-Gutiérrez S
- Issue date: 2022
- ESAT-6 regulates autophagous response through SOD-2 and as a result induces intracellular survival of Mycobacterium bovis BCG.
- Authors: Yabaji SM, Dhamija E, Mishra AK, Srivastava KK
- Issue date: 2020 Oct
- The non-pathogenic mycobacteria M. smegmatis and M. fortuitum induce rapid host cell apoptosis via a caspase-3 and TNF dependent pathway.
- Authors: Bohsali A, Abdalla H, Velmurugan K, Briken V
- Issue date: 2010 Sep 10
- Mechanisms of persistence of mycobacteria.
- Authors: Britton WJ, Roche PW, Winter N
- Issue date: 1994 Aug