In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses.
dc.contributor.author | Regus-Leidig, Hanna | |
dc.contributor.author | Fuchs, Michaela | |
dc.contributor.author | Löhner, Martina | |
dc.contributor.author | Leist, Sarah R | |
dc.contributor.author | Leal-Ortiz, Sergio | |
dc.contributor.author | Chiodo, Vince A | |
dc.contributor.author | Hauswirth, William W | |
dc.contributor.author | Garner, Craig C | |
dc.contributor.author | Brandstätter, Johann H | |
dc.date.accessioned | 2014-10-10T09:40:19Z | |
dc.date.available | 2014-10-10T09:40:19Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses. 2014, 8:259 Front Cell Neurosci | en |
dc.identifier.issn | 1662-5102 | |
dc.identifier.pmid | 25232303 | |
dc.identifier.doi | 10.3389/fncel.2014.00259 | |
dc.identifier.uri | http://hdl.handle.net/10033/332645 | |
dc.description.abstract | Piccolo is the largest known cytomatrix protein at active zones of chemical synapses. A growing number of studies on conventional chemical synapses assign Piccolo a role in the recruitment and integration of molecules relevant for both endo- and exocytosis of synaptic vesicles, the dynamic assembly of presynaptic F-actin, as well as the proteostasis of presynaptic proteins, yet a direct function in the structural organization of the active zone has not been uncovered in part due to the expression of multiple alternatively spliced isoforms. We recently identified Piccolino, a Piccolo splice variant specifically expressed in sensory ribbon synapses of the eye and ear. Here we down regulated Piccolino in vivo via an adeno-associated virus-based RNA interference approach and explored the impact on the presynaptic structure of mouse photoreceptor ribbon synapses. Detailed immunocytochemical light and electron microscopical analysis of Piccolino knockdown in photoreceptors revealed a hitherto undescribed photoreceptor ribbon synaptic phenotype with striking morphological changes of synaptic ribbon ultrastructure. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to Frontiers in cellular neuroscience | en |
dc.title | In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses. | en |
dc.type | Article | en |
dc.identifier.journal | Frontiers in cellular neuroscience | en |
refterms.dateFOA | 2018-06-13T15:14:15Z | |
html.description.abstract | Piccolo is the largest known cytomatrix protein at active zones of chemical synapses. A growing number of studies on conventional chemical synapses assign Piccolo a role in the recruitment and integration of molecules relevant for both endo- and exocytosis of synaptic vesicles, the dynamic assembly of presynaptic F-actin, as well as the proteostasis of presynaptic proteins, yet a direct function in the structural organization of the active zone has not been uncovered in part due to the expression of multiple alternatively spliced isoforms. We recently identified Piccolino, a Piccolo splice variant specifically expressed in sensory ribbon synapses of the eye and ear. Here we down regulated Piccolino in vivo via an adeno-associated virus-based RNA interference approach and explored the impact on the presynaptic structure of mouse photoreceptor ribbon synapses. Detailed immunocytochemical light and electron microscopical analysis of Piccolino knockdown in photoreceptors revealed a hitherto undescribed photoreceptor ribbon synaptic phenotype with striking morphological changes of synaptic ribbon ultrastructure. |