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Authors
Roesner, Lennart MMielke, Christian
Faehnrich, Silke
Merkhoffer, Yvonne
Dittmar, Kurt E J
Drexler, Hans G
Dirks, Wilhelm G
Issue Date
2014
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Show full item recordAbstract
Mutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers.Citation
Localization of MLH3 at the centrosomes. 2014, 15 (8):13932-7 Int J Mol SciPubMed ID
25116689Type
ArticleLanguage
enISSN
1422-0067ae974a485f413a2113503eed53cd6c53
10.3390/ijms150813932
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