Comparing the anterior nare bacterial community of two discrete human populations using Illumina amplicon sequencing.
supplemtal figures and tables
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Oxley, Andrew P A
Wos-Oxley, Melissa L
Pieper, Dietmar H
MetadataShow full item record
AbstractThe anterior nares are an important reservoir for opportunistic pathogens and commensal microorganisms. A barcoded Illumina paired-end sequencing method targeting the 16S ribosomal RNA V1-2 hypervariable region was developed to compare the bacterial diversity of the anterior nares across distinct human populations (volunteers from Germany vs a Babongo Pygmy tribe, Africa). Of the 251 phylotypes detected, 231 could be classified to the genus level and 109 to the species level, including the unambiguous identification of the ubiquitous Staphylococcus aureus and Moraxella catarrhalis. The global bacterial community of both adult populations revealed that they shared 85% of the phylotypes, suggesting that our global bacterial communities have likely been with us for thousands of years. Of the 34 phylotypes unique to the non-westernized population, most were related to members within the suborder Micrococcineae. There was an even more overwelming distinction between children and adults of the same population, suggesting a progression of a childhood community of high-diversity comprising species of Moraxellaceae and Streptococcaceae to an adult community of lower diversity comprising species of Propionibacteriaceae, Clostridiales Incertae Sedis XI, Corynebacteriaceae and Staphylococcaceae. Thus, age was a stronger factor for accounting for differing bacterial assemblages than the origin of the human population sampled.
CitationComparing the anterior nare bacterial community of two discrete human populations using Illumina amplicon sequencing. 2014, 16 (9):2939-52 Environ. Microbiol.
AffiliationMicrobial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
The following license files are associated with this item:
- A poke into the diversity and associations within human anterior nare microbial communities.
- Authors: Wos-Oxley ML, Plumeier I, von Eiff C, Taudien S, Platzer M, Vilchez-Vargas R, Becker K, Pieper DH
- Issue date: 2010 Jul
- Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform.
- Authors: Castelino M, Eyre S, Moat J, Fox G, Martin P, Ho P, Upton M, Barton A
- Issue date: 2017 Jan 21
- Next-generation sequencing reveals significant bacterial diversity of botrytized wine.
- Authors: Bokulich NA, Joseph CM, Allen G, Benson AK, Mills DA
- Issue date: 2012
- Analysis of the upper respiratory tract microbiotas as the source of the lung and gastric microbiotas in healthy individuals.
- Authors: Bassis CM, Erb-Downward JR, Dickson RP, Freeman CM, Schmidt TM, Young VB, Beck JM, Curtis JL, Huffnagle GB
- Issue date: 2015 Mar 3
- Validating T-RFLP as a sensitive and high-throughput approach to assess bacterial diversity patterns in human anterior nares.
- Authors: Camarinha-Silva A, Wos-Oxley ML, Jáuregui R, Becker K, Pieper DH
- Issue date: 2012 Jan