Biosynthesis of magnetic nanostructures in a foreign organism by transfer of bacterial magnetosome gene clusters.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Plitzko, Jürgen M
MetadataShow full item record
AbstractThe synthetic production of monodisperse single magnetic domain nanoparticles at ambient temperature is challenging. In nature, magnetosomes--membrane-bound magnetic nanocrystals with unprecedented magnetic properties--can be biomineralized by magnetotactic bacteria. However, these microbes are difficult to handle. Expression of the underlying biosynthetic pathway from these fastidious microorganisms within other organisms could therefore greatly expand their nanotechnological and biomedical applications. So far, this has been hindered by the structural and genetic complexity of the magnetosome organelle and insufficient knowledge of the biosynthetic functions involved. Here, we show that the ability to biomineralize highly ordered magnetic nanostructures can be transferred to a foreign recipient. Expression of a minimal set of genes from the magnetotactic bacterium Magnetospirillum gryphiswaldense resulted in magnetosome biosynthesis within the photosynthetic model organism Rhodospirillum rubrum. Our findings will enable the sustainable production of tailored magnetic nanostructures in biotechnologically relevant hosts and represent a step towards the endogenous magnetization of various organisms by synthetic biology.
CitationBiosynthesis of magnetic nanostructures in a foreign organism by transfer of bacterial magnetosome gene clusters. 2014, 9 (3):193-7 Nat Nanotechnol
AffiliationLudwig-Maximilians-Universität München, Department of Biology I, Großhaderner Straße 2-4, 82152 Martinsried, Germany.
The following license files are associated with this item:
- Overproduction of Magnetosomes by Genomic Amplification of Biosynthesis-Related Gene Clusters in a Magnetotactic Bacterium.
- Authors: Lohße A, Kolinko I, Raschdorf O, Uebe R, Borg S, Brachmann A, Plitzko JM, Müller R, Zhang Y, Schüler D
- Issue date: 2016 May 15
- Nanoparticle biosynthesis: An accommodating host.
- Authors: Staniland S
- Issue date: 2014 Mar
- Probing the Nanostructure and Arrangement of Bacterial Magnetosomes by Small-Angle X-Ray Scattering.
- Authors: Rosenfeldt S, Riese CN, Mickoleit F, Schüler D, Schenk AS
- Issue date: 2019 Dec 15
- New vectors for chromosomal integration enable high-level constitutive or inducible magnetosome expression of fusion proteins in Magnetospirillum gryphiswaldense.
- Authors: Borg S, Hofmann J, Pollithy A, Lang C, Schüler D
- Issue date: 2014 Apr
- Comprehensive genetic dissection of the magnetosome gene island reveals the step-wise assembly of a prokaryotic organelle.
- Authors: Murat D, Quinlan A, Vali H, Komeili A
- Issue date: 2010 Mar 23